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Creatine Assay Kit (Colorimetric/Fluorometric) (ab65339) provides an accurate, convenient measure of creatine in a variety of biological samples. In the assay, creatine is enzymatically converted to sarcosine which is then specifically oxidized to generate a product that converts a colorless probe to an intensely red color (?max = 570 nm), and highly fluorescent (Ex/Em = 538/587 nm) product. Creatine is therefore easily detected by either colorimetric or fluorometric methods. Detection range 0.001–10 mM Creatine.
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Creatine is an endogenous compound whose function is to maintain a high ATP/ADP ratio, by way of its phosphorylated form and creatine kinase. Creatine supplementation has been used in the treatment of muscular, neurological and neurodegenerative diseases, as well as a sport performance enhancer. Detection of creatine level has wide applications in research and development.
|Creatinase (Lyophilized)||Blue||1 vial|
|Creatine Assay Buffer||WM,||1 x 25ml|
|Creatine Enzyme Mix (Lyophilized)||Green||1 vial|
|Creatine Probe (in DMSO)||Red||1 x 200µl|
|Creatine Standard (10 µmol) (Lyophilized)||Yellow||1 vial|
Our Abpromise guarantee covers the use of ab65339 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Functional Studies||Use at an assay dependent dilution.|
Creatine measured in cell lysates showing quantity (nmol) per 106 cells
Creatine measured in biologicals showing concentration (micromolar)
ab65339 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"