Anti-Cre recombinase抗体(ab40011)

概述

  • 产品名称
    Anti-Cre recombinase抗体
    参阅全部 Cre recombinase 一抗
  • 描述
    兔多克隆抗体to Cre recombinase
  • 经测试应用
    适用于: WBmore details
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 250 to the C-terminus of Bacteriophage P1 Cre recombinase.

    (Peptide available as ab41101.)

  • 阳性对照
    • This antibody gave a positive signal against the Cre Recombinase Protein.

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液
    Preservative: 0.02% Sodium Azide
    Constituents: 1% BSA, PBS, pH 7.4
  • Concentration information loading...
  • 纯度
    Immunogen affinity purified
  • 克隆
    多克隆
  • 同种型
    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab40011 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Detects a band of approximately 38 kDa (predicted molecular weight: 38 kDa).

靶标

  • 相关性
    Cre-recombinase is a 38 kDa DNA recombinase derived from the P1 bacteriophage. It is highly specific for a 34 bp DNA sequence (loxP) found in P1 DNA. It catalyzes site-specific recombination between two 34-base-pair LOXP sites. Its role is to maintain the phage genome as a monomeric unit-copy plasmid in the lysogenic state. It is homotetramer when bound to DNA and belongs to the "phage" integrase family.
  • 别名
    • Cre antibody
    • Cyclization recombinase antibody
    • Recombinase cre antibody

图片

  • All lanes : Anti-Cre recombinase antibody (ab40011) at 1/100 dilution

    Lane 1 : Hek293 cell lysate expressing Cre-recombinase
    Lane 2 : Control vector

    Lysates/proteins at 10 µg per lane.

    Secondary
    goat-anti Rabbit HRP conjugated at 1/3000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 38 kDa
    Observed band size : 34 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 55 kDa (possible non-specific binding).

    Exposure time : 1 minute

    This image is courtesy of Britta Eickholt, Univ London Kings Coll, United Kingdom

  • All lanes : Anti-Cre recombinase antibody (ab40011) at 1 µg/ml

    Lane 1 : HEK 293 Empty Vector Negative Control
    Lane 2 : HEK 293 Overexpressing Cre Recombinase

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 38 kDa
    Observed band size : 38 kDa


    Exposure time : 4 minutes
  • Anti-Cre recombinase antibody (ab40011) at 1 µg/ml + Cre Recombinase Protein at 1 µg

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 38 kDa
    Observed band size : 38 kDa


    Exposure time : 20 minutes

实验方案

文献

This product has been referenced in:
  • Plummer PN  et al. MicroRNAs regulate tumor angiogenesis modulated by endothelial progenitor cells. Cancer Res 73:341-52 (2013). IHC ; Mouse . Read more (PubMed: 22836757) »

See 1 Publication for this product

客户评价及客户问答

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Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (Liver)
Specification
Liver
Fixative
Paraformaldehyde
Permeabilization
Yes - PBS 0,3% triton 100 X
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
Username

Mrs. Elisa Pedone

Verified customer

提交于 Jun 18 2012

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (Retina)
Permeabilization
Yes - 0.3% Triton
Specification
Retina
Blocking step
Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 0.5% · Temperature: 23°C
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

提交于 Feb 06 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (expression of Cre-recombinase in Hek293 cells)
Specification
expression of Cre-recombinase in Hek293 cells
Gel Running Conditions
Reduced Denaturing (8%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1µg/mL · Temperature: 37°C
Username

Dr. Britta Eickholt

Verified customer

提交于 May 10 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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