Anti-Cre recombinase抗体(ab40011)

概述

  • 产品名称Anti-Cre recombinase抗体
    参阅全部 Cre recombinase 一抗
  • 描述
    兔多克隆抗体to Cre recombinase
  • 经测试应用适用于: WBmore details
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 250 to the C-terminus of Bacteriophage P1 Cre recombinase.

    (Peptide available as ab41101.)

  • 阳性对照
    • This antibody gave a positive signal against the Cre Recombinase Protein.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液Preservative: 0.02% Sodium Azide
    Constituents: 1% BSA, PBS, pH 7.4
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab40011 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Detects a band of approximately 38 kDa (predicted molecular weight: 38 kDa).

靶标

  • 相关性Cre-recombinase is a 38 kDa DNA recombinase derived from the P1 bacteriophage. It is highly specific for a 34 bp DNA sequence (loxP) found in P1 DNA. It catalyzes site-specific recombination between two 34-base-pair LOXP sites. Its role is to maintain the phage genome as a monomeric unit-copy plasmid in the lysogenic state. It is homotetramer when bound to DNA and belongs to the "phage" integrase family.
  • 别名
    • Cre antibody
    • Cyclization recombinase antibody
    • Recombinase cre antibody

Anti-Cre recombinase antibody 图像

  • All lanes : Anti-Cre recombinase antibody (ab40011) at 1/100 dilution

    Lane 1 : Hek293 cell lysate expressing Cre-recombinase
    Lane 2 : Control vector

    Lysates/proteins at 10 µg per lane.

    Secondary
    goat-anti Rabbit HRP conjugated at 1/3000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 38 kDa
    Observed band size : 34 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 55 kDa (possible non-specific binding).

    Exposure time : 1 minute

    This image is courtesy of Britta Eickholt, Univ London Kings Coll, United Kingdom

  • All lanes : Anti-Cre recombinase antibody (ab40011) at 1 µg/ml

    Lane 1 : HEK 293 Empty Vector Negative Control
    Lane 2 : HEK 293 Overexpressing Cre Recombinase

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 38 kDa
    Observed band size : 38 kDa


    Exposure time : 4 minutes
  • Anti-Cre recombinase antibody (ab40011) at 1 µg/ml + Cre Recombinase Protein at 1 µg

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 38 kDa
    Observed band size : 38 kDa


    Exposure time : 20 minutes

Anti-Cre recombinase antibody (ab40011)参考文献

This product has been referenced in:
  • Plummer PN  et al. MicroRNAs regulate tumor angiogenesis modulated by endothelial progenitor cells. Cancer Res 73:341-52 (2013). IHC ; Mouse . Read more (PubMed: 22836757) »

See 1 Publication for this product

Product Wall

Thank you for contacting us.

Perhaps these are the antibodies customer is looking for;

http://www.abcam.com/cre-recombinase-antibody-723-ab24607.html

http://www.abcam.com/cre-recombinase-antibody-723-biotin-ab24580.html
<...

Read More

Thank you for your reply.


In answer to your questions:

Q1) Membranes are blocked for 1 hour at RT

Q2) 1ug of Cre Recombinase Protein was loaded on the gel

Q3) Samples are boiled 10 min prior to loading

...

Read More

Thank you for contacting Abcam regarding ab40011.


The WB images on the datasheet were representative of two slightly differentconditions.


The first image, was submitted by a customer Abreview. The only information we have re...

Read More

Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.

I am sorry this product did not perform as stated on the datasheet and for the inconvenience this...

Read More
Application Immunocytochemistry/ Immunofluorescence
Sample Mouse Cell (Liver)
Specification Liver
Fixative Paraformaldehyde
Permeabilization Yes - PBS 0,3% triton 100 X
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
Username

Mrs. Elisa Pedone

Verified customer

提交于 Jun 18 2012

I am very pleased to hear you would like to accept our offer and test ab40011 in IHC on frozen tissue. This code will give you:one free primary antibody before the expiration date. To redeem this offer, please submit an Abreview for ab40011and include ...

Read More

redback.

I am very sorry this antibody did not work in IF. Once the results have been submitted as an Abreview our Scientific Support team will study the protocol to try to optimise it and obtain the expected results from the antibody.
<...

Read More

Thank you for your reply.

DISCOUNT CODE: XXXXXX

Expiration date: 10-Sep-12

I am very pleased to hear you would like to accept our offer and test ab40011 with ICC/IF. This code will give you 1 free primary antibody before th...

Read More

Thank you for contacting us.

Unfortunately ab40011 has not been tested with immunofluorescence. It doesn’t mean it won’t work in this application, but we don’t have data to support it.

If you are interested in test...

Read More

Thank you for bringing this to our attention and for completing the troubleshooting form. Your sample preparation and protocol are correct. I agree that the antibody appears to be the problem. We do not have a different lot, so I suggest replacing this...

Read More

1-10 of 21 Abreviews or Q&A

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"