Anti-Cre recombinase抗体[7.23] (ab24607)

概述

  • 产品名称
    Anti-Cre recombinase抗体[7.23]
    参阅全部 Cre recombinase 一抗
  • 描述
    小鼠单克隆抗体[7.23] to Cre recombinase
  • 特异性
    Current Batch not validated for Immunohistochemistry.
  • 经测试应用
    适用于: WBmore details
  • 免疫原

    Full length native protein (affinity purified).

  • 常规说明


    This antibody can be used to determine the success of gene manipulations in transgenic animals.

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液
    Preservative: 0.03% Thimerosal (merthiolate)
    Constituents: PBS, 50% Glycerol
  • Concentration information loading...
  • 纯度
    Protein G purified
  • Primary antibody说明
    This antibody can be used to determine the success of gene manipulations in transgenic animals.
  • 克隆
    单克隆
  • 克隆编号
    7.23
  • 同种型
    IgG1
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab24607 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500. Detects a band of approximately 34 kDa (predicted molecular weight: 38 kDa).

靶标

  • 相关性
    Cre-recombinase is a 38 kDa DNA recombinase derived from the P1 bacteriophage. It is highly specific for a 34 bp DNA sequence (loxP) found in P1 DNA. It catalyzes site-specific recombination between two 34-base-pair LOXP sites. Its role is to maintain the phage genome as a monomeric unit-copy plasmid in the lysogenic state. It is homotetramer when bound to DNA and belongs to the "phage" integrase family.
  • 别名
    • Cre antibody
    • Cyclization recombinase antibody
    • Recombinase cre antibody

图片

  • All lanes : Anti-Cre recombinase antibody [7.23] (ab24607) at 1/1000 dilution

    Lane 1 : Escherichia coli expressing Cre-recombinase
    Lane 2 : Hek cell expressing control plasmid

    Lysates/proteins at 10 µg per lane.

    Secondary
    goat anti-mouse HRP conjugated at 1/3000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 38 kDa
    Observed band size : 34 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 50&80 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 1 minute

    This image is courtesy of Britta Eickholt, Univ London Kings Coll, United Kingdom

实验方案

文献

This product has been referenced in:

See all 12 Publications for this product

客户评价及客户问答

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Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunohistochemistry free floating
Sample
Rat Tissue sections (Brain tissue (Inferior colliculus and Medial Genic)
Specification
Brain tissue (Inferior colliculus and Medial Genic
Username

Abcam user community

Verified customer

提交于 Jun 14 2017

Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (mouse embryonic spinal cord (cryosections))
Permeabilization
Yes - 0.1%Triton X-100
Specification
mouse embryonic spinal cord (cryosections)
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 20°C
Fixative
Paraformaldehyde
Username

Dr. Frederic Clotman

Verified customer

提交于 Nov 19 2015

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (HeLa Cells)
Loading amount
25 µg
Specification
HeLa Cells
Gel Running Conditions
Non-reduced Denaturing (10% gel)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Username

Abcam user community

Verified customer

提交于 Jun 08 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Escherichia coli Cell lysate - whole cell (vector expressing Cre-recombinase)
Specification
vector expressing Cre-recombinase
Gel Running Conditions
Reduced Denaturing (8%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 37°C
Username

Dr. Britta Eickholt

Verified customer

提交于 May 10 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (retina)
Specification
retina
Fixative
Paraformaldehyde
Permeabilization
No
Blocking step
Serum as blocking agent for 1 hour(s) and 30 minute(s) · Concentration: 5% · Temperature: 20°C
Username

Abcam user community

Verified customer

提交于 Jun 22 2009

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (inner ear)
Specification
inner ear
Fixative
Paraformaldehyde
Permeabilization
Yes - 0,03% triton10X
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 20% · Temperature: 22°C
Username

Dr. Vincent Michel

Verified customer

提交于 Jun 22 2009

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (HEK293 cells)
Loading amount
10 µg
Specification
HEK293 cells
Gel Running Conditions
Reduced Denaturing (12% gel)
Blocking step
Milk as blocking agent for 3 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 22°C
Username

Abcam user community

Verified customer

提交于 Apr 17 2009

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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