重组Anti-CRCP抗体[EPR9670(B)] (ab139264)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR9670(B)] to CRCP
- Suitable for: WB, IHC-P, ICC/IF, IP
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
概述
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产品名称
Anti-CRCP抗体[EPR9670(B)]
参阅全部 CRCP 一抗 -
描述
兔单克隆抗体[EPR9670(B)] to CRCP -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-P, ICC/IF, IPmore details -
种属反应性
与反应: Mouse, Rat, Human -
免疫原
Synthetic peptide within Human CRCP aa 1-100 (N terminal). The exact sequence is proprietary.
Database link: O75575 -
阳性对照
- WB: HeLa and Jurkat whole cell lysate (ab7899). Human prostate and ovary cancer tissue lysates. Mouse kidney and rat brain tissue lysates. IHC-P: Human ovarian carcinoma and breast tissues. ICC/IF: LnCap and HeLa cells. IP: Human prostate cancer tissue lysate.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at -20ºC. -
存储溶液
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 0.05% BSA, 59% PBS -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR9670(B) -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab139264于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
1/1000 - 1/10000. Predicted molecular weight: 14 kDa.
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IHC-P |
1/200. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
See IHC antigen retrieval protocols. For unpurified use at 1/100. |
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ICC/IF |
1/250.
For unpurified use at 1/100. |
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IP |
1/50 - 1/100.
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说明 |
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WB
1/1000 - 1/10000. Predicted molecular weight: 14 kDa. |
IHC-P
1/200. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. See IHC antigen retrieval protocols. For unpurified use at 1/100. |
ICC/IF
1/250. For unpurified use at 1/100. |
IP
1/50 - 1/100. |
靶标
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功能
DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Specific peripheric component of RNA polymerase III which synthesizes small RNAs, such as 5S rRNA and tRNAs. Plays a key role in sensing and limiting infection by intracellular bacteria and DNA viruses. Acts as nuclear and cytosolic DNA sensor involved in innate immune response. Can sense non-self dsDNA that serves as template for transcription into dsRNA. The non-self RNA polymerase III transcripts induce type I interferon and NF- Kappa-B through the RIG-I pathway.
Accessory protein for the calcitonin gene-related peptide (CGRP) receptor. It modulates CGRP responsiveness in a variety of tissues. -
组织特异性
Ubiquitous. Most prevalent in testis. -
序列相似性
Belongs to the eukaryotic RPC9 RNA polymerase subunit family. -
细胞定位
Nucleus. Cell membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 27297 Human
- Entrez Gene: 12909 Mouse
- Entrez Gene: 114205 Rat
- Omim: 606121 Human
- SwissProt: O75575 Human
- SwissProt: O35427 Mouse
- SwissProt: Q8VHM6 Rat
- Unigene: 300684 Human
see all -
别名
- Calcitonin gene related peptide receptor component antibody
- Calcitonin gene related peptide receptor component protein antibody
- Calcitonin gene-related peptide-receptor component protein antibody
see all
图片
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All lanes : Anti-CRCP antibody [EPR9670(B)] (ab139264) at 1/5000 dilution (unpurified)
Lane 1 : Jurkat cell lysate
Lane 2 : Mouse kidney tissue lysate
Lane 3 : Rat brain tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution
Predicted band size: 14 kDa
Observed band size: 20 kDa why is the actual band size different from the predicted?Blocking buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM/TBST.
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All lanes : Anti-CRCP antibody [EPR9670(B)] (ab139264) at 1/10000 dilution (purified)
Lane 1 : Jurkat cell lysate
Lane 2 : Mouse kidney tissue lysate
Lane 3 : Rat brain tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution
Predicted band size: 14 kDa
Observed band size: 20 kDa why is the actual band size different from the predicted?Blocking buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM/TBST.
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All lanes : Anti-CRCP antibody [EPR9670(B)] (ab139264) at 1/1000 dilution (unpurified)
Lane 1 : Human ovary cancer lysate
Lane 2 : HeLa lysate
Lane 3 : Jurkat lysate
Lane 4 : Human prostate cancer lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 14 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast tissue labelling CRCP with unpurified ab139264 at 1/100. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. A prediluted HRP-polymer conjugated anti-rabbit IgG was used as the secondary antibody. Counterstained with Hematoxylin.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast tissue labelling CRCP with purified ab139264 at 1/200. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. A prediluted HRP-polymer conjugated anti-rabbit IgG was used as the secondary antibody. Counterstained with Hematoxylin.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast tissue labelling CRCP with unpurified ab139264 at 1/50.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human ovarian carcinoma tissue labelling CRCP with unpurified ab139264 at 1/50.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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Immunocytochemsitry/Immunofluorescence analysis of LnCap cells labelling CRCP (red) with unpurified ab139264 at 1/100. Cells were fixed with 4% paraformaldehyde. An Alexa Fluor® 555-conjugated goat anti-rabbit IgG (1/200) was used as the secondary antibody. Counterstained with DAPI (blue).
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Immunocytochemsitry/Immunofluorescence analysis of LnCap cells labelling CRCP (red) with purified ab139264 at 1/250. Cells were fixed with 4% paraformaldehyde. An Alexa Fluor® 555-conjugated goat anti-rabbit IgG (1/200) was used as the secondary antibody. Counterstained with DAPI (blue).
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Immunocytochemistry/Immunofluorescence analysis of HeLa cells labelling CRCP with unpurified ab139264 at 1/100.
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ab139264 (unpurified) at 1/50 immunoprecipitating CRCP in human prostate cancer tissue lysate (Lane 1). Lane 2 - PBS. For western blotting, a HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG was used as the secondary antibody (1/1500).
Blocking buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM /TBST.
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ab139264 (purified) at 1/100 immunoprecipitating CRCP in human prostate cancer tissue lysate (Lane 1). Lane 2 - PBS. For western blotting, a HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG was used as the secondary antibody (1/1500).
Blocking buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM /TBST.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (2)
ab139264 被引用在 2 文献中.
- Mounir MMF et al. Regeneration of Neural Networks in Immature Teeth with Non-Vital Pulp Following a Novel Regenerative Procedure. Int J Stem Cells 12:410-418 (2019). PubMed: 31658509
- Bao Y et al. Topical treatment with Xiaozheng Zhitong Paste alleviates bone cancer pain by inhibiting proteinase-activated receptor 2 signaling pathway. Oncol Rep 34:1449-59 (2015). PubMed: 26133236