• 产品名称Anti-CRBN抗体
    参阅全部 CRBN 一抗
  • 描述
    兔多克隆抗体to CRBN
  • 经测试应用适用于: ICC/IF, WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Zebrafish
  • 免疫原

    Synthetic peptide, corresponding to a region within N terminal amino acids 35-84 (DQDSKEAKKP NIINFDTSLP TSHTYLGADM EEFHGRTLHD DDSCQVIPVL) of Human CRBN (NP_057386).

  • 阳性对照
    • COLO205 cell IF/ICC: SKNSH cell line


  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液Preservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 克隆多克隆
  • 同种型IgG
  • 研究领域


Our Abpromise guarantee covers the use of ab98992 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use a concentration of 5 µg/ml.
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 50 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.


  • 功能Component of some DCX (DDB1-CUL4-X-box) E3 protein ligase complex, a complex that mediates the ubiquitination and subsequent proteasomal degradation of target proteins and is required for limb outgrowth and expression of the fibroblast growth factor FGF8. In the complex, may act as a substrate receptor. Regulates the assembly and neuronal surface expression of large-conductance calcium-activated potassium channels in brain regions involved in memory and learning via its interaction with KCNT1.
  • 组织特异性Widely expressed. Highly expressed in brain.
  • 通路Protein modification; protein ubiquitination.
  • 疾病相关Defects in CRBN are the cause of mental retardation autosomal recessive type 2A (MRT2A) [MIM:607417]. MRT2A patients display mild mental retardation with a standard IQ ranged from 50 to 70. IQ scores are lower in males than females. Developmental milestones are mildly delayed. There are no dysmorphic or autistic features. Non-syndromic mental retardation patients do not manifest other clinical signs.
  • 序列相似性Belongs to the CRBN family.
    Contains 1 Lon domain.
  • 翻译后修饰Ubiquitinated, ubiquitination is mediated by its own DCX protein ligase complex.
  • 细胞定位Cytoplasm. Nucleus. Membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • 2610203G15Rik antibody
    • 2900045O07Rik antibody
    • AF229032 antibody
    • AW108261 antibody
    • Cereblon antibody
    • Crbn antibody
    • CRBN_HUMAN antibody
    • DKFZp781K0715 antibody
    • MGC27358 antibody
    • MRT2A antibody
    • OTTHUMP00000209555 antibody
    • piL antibody
    • Protein cereblon antibody
    • Protein x 0001 antibody
    see all

Anti-CRBN antibody 图像

  • ICC/IF image of ab98992 stained SKNSH cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab98992, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Anti-CRBN antibody (ab98992) at 1 µg/ml + COLO205 cell lysate at 10 µg

    Predicted band size : 50 kDa

Anti-CRBN antibody (ab98992)参考文献

ab98992 has not yet been referenced specifically in any publications.

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Expiration date: May 30th, 2012

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Application Western blot
Sample Mouse Purified protein (Purified protein of HEK293(mouse CRBN-His6 overexp)
Gel Running Conditions Reduced Denaturing (10 % gel)
Loading amount 3 µg
Specification Purified protein of HEK293(mouse CRBN-His6 overexp
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

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Verified customer

提交于 Oct 30 2015

Application Western blot
Loading amount 30 µg
Gel Running Conditions Reduced Denaturing
Sample Human Cell lysate - whole cell (Myeloma cell)
Specification Myeloma cell
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 25°C

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Verified customer

提交于 Feb 05 2014