概述

  • 产品名称Anti-CRABP2抗体
    参阅全部 CRABP2 一抗
  • 描述
    兔多克隆抗体to CRABP2
  • 特异性ab74365 detects endogenous levels of total CRABP2 protein.
  • 经测试应用适用于: ICC, WB, Indirect ELISA, IHC-Pmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    A synthesized peptide derived from an internal region of human CRABP2.

  • 阳性对照
    • Extracts from HT-29 cells.

性能

应用

Our Abpromise guarantee covers the use of ab74365 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC Use at an assay dependent concentration.
WB 1/500 - 1/1000. Predicted molecular weight: 16 kDa.
Indirect ELISA 1/20000.
IHC-P 1/100.

靶标

  • 功能Transports retinoic acid to the nucleus. Regulates the access of retinoic acid to the nuclear retinoic acid receptors.
  • 序列相似性Belongs to the calycin superfamily. Fatty-acid binding protein (FABP) family.
  • 结构域Forms a beta-barrel structure that accommodates hydrophobic ligands in its interior.
  • 细胞定位Cytoplasm. Nucleus. Upon ligand binding, a conformation change exposes a nuclear localization motif and the protein is transported into the nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Cellular retinoic acid binding protein 2 antibody
    • Cellular retinoic acid binding protein II antibody
    • Cellular retinoic acid-binding protein 2 antibody
    • Cellular retinoic acid-binding protein II antibody
    • CRABP II antibody
    • CRABP-II antibody
    • Crabp2 antibody
    • CRABPII antibody
    • RABP2_HUMAN antibody
    • RBP6 antibody
    • Rretinoic acid-binding protein, cellular, type II antibody
    see all

Anti-CRABP2 antibody 图像

  • All lanes : Anti-CRABP2 antibody (ab74365) at 1/500 dilution

    Lane 1 : extracts from HT-29 cells
    Lane 2 : extracts from HT-29 cells with immunizing peptide at 10 µg

    Lysates/proteins at 30 µg per lane.


    Predicted band size : 16 kDa
    Observed band size : 16 kDa
  • ab74365 staining CRABP2 in Human Schwann Cells by ICC (Immunocytochemistry). Cells were fixed with formaldehyde, permeabilized with 0.1% Triton in PBS and blocked with 1% BSA for 1 hour at 25°C. Samples were incubated with primary antibody (1/100 in PBS + 1% BSA) for 1 hour at 25°C. An Biotin-conjugated Goat anti-rabbit IgG polyclonal (1/100) was used as the secondary antibody.

    See Abreview

Anti-CRABP2 antibody (ab74365)参考文献

This product has been referenced in:
  • Yao PL  et al. Activation of peroxisome proliferator-activated receptor-ß/d (PPAR-ß/d) inhibits human breast cancer cell line tumorigenicity. Mol Cancer Ther 13:1008-17 (2014). Human . Read more (PubMed: 24464939) »
  • Morris DR & Levenson CW Zinc regulation of transcriptional activity during retinoic acid-induced neuronal differentiation. J Nutr Biochem 24:1940-4 (2013). WB ; Human . Read more (PubMed: 24029070) »

See all 3 Publications for this product

Product Wall

Thank you for contacting us.

The immuogen is from amino acid 50 - 100 of human CRABP2; http://www.uniprot.org/uniprot/P29373

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice o...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry
Sample Human Cultured Cells (Schwann Cell (T265))
Specification Schwann Cell (T265)
Fixative Formaldehyde
Permeabilization Yes - 0.1% Triton/PBS
Blocking step BSA as blocking agent for 20 minute(s) · Concentration: 1% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 May 01 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (adult testis)
Specification adult testis
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: citric acid buffer
Permeabilization Yes - 0.1% Triton-100 in 1x PBS
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 Mar 23 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"