概述

  • 产品名称Anti-CRABP1抗体[C-1]
    参阅全部 CRABP1 一抗
  • 描述
    小鼠单克隆抗体[C-1] to CRABP1
  • 特异性Detects cellular Retinoic Acid Binding Protein 1 (CRABP 1). This antibody does not cross-react with Cellular Retinol Binding Protein (CRBP), performic acid oxidized CRBP, interphotoreceptor retinoid binding protein or retinol binding protein. No reaction has been seen for chameleon, goldfish and frog CRABP1.
  • 经测试应用适用于: IHC-P, ICC/IF, ELISA, IHC-Fr, WB, Flow Cytmore details
  • 种属反应性
    与反应: Mouse, Rat, Rabbit, Chicken, Cow, Cat, Human, Non Human Primates, Reptiles
    预测可用于: Bird不与反应: Goldfish
  • 免疫原

    Full length protein corresponding to Cow CRABP1. Oxidized CRABPI from Bovine retina.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液Preservative: 0.05% Sodium azide
    Constituent: PBS
  • Concentration information loading...
  • 纯度Ascites
  • Primary antibody说明Retinoic acid (RA), a metabolite of vitamin A, plays an important role in growth and differentiation by modulation of expression of certain genes. RA functions through its interaction with the nuclear protein, retinoic acid receptor (RAR) and the 9-cis RA with retinoid X receptor (RXR). The Cellular Retinoic Acid Binding Proteins (CRABP) bind RA with a high affinity and belong to the family of fatty acid binding proteins. Two forms of CRABP have been characterized, CRABP 1 and CRABP 2 which share ~75% sequence conservation. The main role of CRABP 1 may be to protect retinoids from non-specific dehydrogenases and direct them to specific enzymes. The precise role of CRABP 2 is still poorly understood but it seems to play a role in the interaction of ligands (RA, 9-cis RA) with nuclear receptors (RAR/RXR).
  • 克隆单克隆
  • 克隆编号C-1
  • 同种型IgG2b
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab2816 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P 1/1000.
ICC/IF 1/100 - 1/200.
ELISA Use at an assay dependent concentration.
IHC-Fr 1/250.
WB 1/1000. Detects a band of approximately 16 kDa (predicted molecular weight: 15 kDa).
Flow Cyt 1/100.

靶标

Anti-CRABP1 antibody [C-1] 图像

  • Immunocytochemistry/Immunofluorescence analysis of CRABPI shows staining in HeLa cells. CRABPI staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were incubated without (control) or with ab2816 (1:200) overnight at 4°C, washed with PBS and incubated with a DyLight-488 conjugated goat anti-mouse secondary antibody. Images were taken at 60X magnification.

  • Immunocytochemistry/Immunofluorescence analysis of CRABPI shows staining in MCF-7 cells. CRABPI staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were incubated without (control) or with ab2816 (1:100) overnight at 4°C, washed with PBS and incubated with a DyLight-488 conjugated goat anti-mouse secondary antibody. Images were taken at 60X magnification.

  • Immunocytochemistry/Immunofluorescence analysis of CRABPI shows staining in NIH-3T3 cells. CRABPI staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were incubated without (control) or with ab2816 (1:200) overnight at 4°C, washed with PBS and incubated with a DyLight-488 conjugated goat anti-mouse secondary antibody. Images were taken at 60X magnification.

  • Anti-CRABP1 antibody [C-1] (ab2816) at 1/1000 dilution + 50ug of human ovary whole cell lysate

    Secondary
    HRP polymer conjugated Rabbit anti-mouse IgG (H&L)
    Developed using the ECL technique

    Predicted band size : 15 kDa
    Observed band size : 16 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 35 kDa (possible non-specific binding).

    Exposure time : 10 seconds

    This image is courtesy of an Abreview submitted by Mr John Andersen

    See Abreview

  • ab2816 (2 µg/ml) staining CRabP1in human thyroid gland using an automated system (DAKO Autostainer Plus). Using this protocol there is strong nuclear and cytoplasmic staining.
    Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer EDTA pH 9.0 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.
  • Overlay histogram showing CHO cells stained with ab2816 (red line). The cells were fixed with 100% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab2816, 1/100 dilution) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (ab91366, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in CHO cells fixed with 4% paraformaldehyde (10 min)/permeabilized in 0.1% PBS-Tween used under the same conditions.

  • Immunohistochemistry was performed on both normal and cancer biopsies of deparaffinized Human thyroid tissue tissues. To expose target proteins heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:200 with a mouse monoclonal antibody recognizing CRABPI ab2816 or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.

  • Immunohistochemistry was performed on both normal and cancer biopsies of deparaffinized Human breast carcinoma tissues. To expose target proteins heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:200 with a mouse monoclonal antibody recognizing CRABPI ab2816 or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.

Anti-CRABP1 antibody [C-1] (ab2816)参考文献

This product has been referenced in:
  • McCready J  et al. Pregnancy-associated breast cancers are driven by differences in adipose stromal cells present during lactation. Breast Cancer Res 16:R2 (2014). IHC-P ; Mouse . Read more (PubMed: 24405573) »
  • Akama K  et al. Proteomic identification of differentially expressed genes during differentiation of cynomolgus monkey (Macaca fascicularis) embryonic stem cells to astrocyte progenitor cells in vitro. Biochim Biophys Acta 1834:601-10 (2013). Read more (PubMed: 23232153) »

See all 11 Publications for this product

Product Wall

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Rat Tissue sections (Brain)
Specification Brain
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citric acid
Permeabilization No
Blocking step BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
Username

Mr. Carl Hobbs

Verified customer

提交于 Dec 27 2012

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (Brain)
Specification Brain
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citric acid
Permeabilization No
Blocking step BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
Username

Mr. Carl Hobbs

Verified customer

提交于 Dec 21 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Sample Mouse Tissue sections (Dermis including follicle)
Specification Dermis including follicle
Fixative Paraformaldehyde
Permeabilization Yes - 0.1% Triton
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 20% · Temperature: 19°C
Username

Abcam user community

Verified customer

提交于 Jan 18 2011

Thank you for your enquiry and your interest in our products. Regarding your inquiry, ab2816 - CRABP1 antibody [C-1] has shown no cross reactivity with the retinoid binding proteins CRPB, performic acid oxidized CRBP, CRALBP, IRBP, RBP, or performic...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (Ovarian carcinoma)
Specification Ovarian carcinoma
Fixative Formaldehyde
Antigen retrieval step None
Blocking step Sniper solution (Biocare Medical) as blocking agent for 20 minute(s) · Concentration: 100
Username

Abcam user community

Verified customer

提交于 Mar 19 2007

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Tissue lysate - whole (Ovary)
Loading amount 50 µg
Specification Ovary
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%
Username

Mr. John Andersen

Verified customer

提交于 Jun 16 2006

Thank you for your enquiry. All the information we have on species cross reactivity is specified on the datasheet, these are updated as soon as any new information is brought to our attention. As far as we are aware, cross reactivity with mouse...

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