概述

  • 产品名称Anti-Cpt1c抗体
    参阅全部 Cpt1c 一抗
  • 描述
    兔多克隆抗体to Cpt1c
  • 经测试应用适用于: IP, WB, IHC-P, ICC/IFmore details
  • 种属反应性
    与反应: Mouse, Human
    预测可用于: Rat
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human Cpt1c.

    (Peptide available as ab99479.)

  • 阳性对照
    • This antibody gave a positive signal in the following lysates: Human Brain Tissue; Human Testis Tissue; Mouse Hippocampus Tissue; Mouse Testis Tissue; HeLa Whole Cell; Jurkat Whole Cell. It also gave a positive signal in FFPE human hippocampus tissue sections. This antibody gave a positive result when used in the following formaldehyde fixed cell lines: PC12

应用

Our Abpromise guarantee covers the use of ab87498 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IP Use a concentration of 5 µg/ml.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 99 kDa (predicted molecular weight: 91 kDa).
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC/IF Use a concentration of 5 - 10 µg/ml.

靶标

  • 组织特异性Expressed predominantly in brain and testis.
  • 通路Lipid metabolism; fatty acid beta-oxidation.
  • 序列相似性Belongs to the carnitine/choline acetyltransferase family.
  • 细胞定位Mitochondrion outer membrane. Cell junction > synapse.
  • Information by UniProt
  • 数据库链接
  • 别名
    • brain isoform antibody
    • Carnitine acyltransferase like protein 1 antibody
    • Carnitine acyltransferase like protein1 antibody
    • Carnitine O palmitoyltransferase 1 brain isoform antibody
    • Carnitine O palmitoyltransferase I, brain isoform antibody
    • Carnitine O-palmitoyltransferase 1 antibody
    • Carnitine O-palmitoyltransferase I antibody
    • Carnitine palmitoyltransferase 1, brain antibody
    • Carnitine palmitoyltransferase 1C antibody
    • Carnitine palmitoyltransferase I related C antibody
    • Carnitine palmitoyltransferase1C antibody
    • CAT L1 antibody
    • CATL 1 antibody
    • CATL1 antibody
    • CPT 1 like pseudogene antibody
    • Cpt 1c antibody
    • CPT 1P antibody
    • CPT I C antibody
    • CPT IC antibody
    • Cpt1 c antibody
    • CPT1 like pseudogene antibody
    • CPT1-B antibody
    • Cpt1c antibody
    • CPT1C_HUMAN antibody
    • CPT1P antibody
    • CPTI-B antibody
    • CPTIC antibody
    see all

Anti-Cpt1c antibody 图像

  • ICC/IF image of ab87498 stained PC12 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab87498 at 10µg/ml overnight at +4°C. The secondary antibody (pseudo-colored green) was Alexa Fluor® 488 goat anti- rabbit (ab150081) IgG (H+L) preadsorbed, used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (pseudo-colored red) at a 1/200 dilution for 1h at room temperature. DAPI was used to stain the cell nuclei (pseudo-colored blue) at a concentration of 1.43µM for 1hour at room temperature.

  • All lanes : Anti-Cpt1c antibody (ab87498) at 1 µg/ml

    Lane 1 : Human brain tissue lysate - total protein (ab29466)
    Lane 2 : Human testis tissue lysate - total protein (ab30257)
    Lane 3 : Hippocampus (Mouse) Tissue Lysate
    Lane 4 : Testis (Mouse) Tissue Lysate
    Lane 5 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 6 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) (ab65484) at 1/3000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 91 kDa
    Observed band size : 99 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 37 kDa,40 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 20 minutes
    The 99 kDa band observed is comparable to the molecular weight seen with our other antibodies to Cpt1c. Abcam welcomes customer feedback.
  • ICC/IF image of ab87498 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab87498, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899 Dylight 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • IHC image of ab87498 staining in human hippocampus formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab87498, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
  • Cpt1c was immunoprecipitated using 0.5mg Mouse Testis tissue lysate, 5µg of Rabbit polyclonal to Cpt1c and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).
    The antibody was incubated under agitation with Protein G beads for 10min, Mouse Testis tissue lysate lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
    Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab87498.
    Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).
    Band: 99kDa, non specific bands - 50 and 55kDa: We are unsure as to the identity of this extra band; Cpt1c.

Anti-Cpt1c antibody (ab87498)参考文献

This product has been referenced in:
  • Xiao G  et al. ATF4 protein deficiency protects against high fructose-induced hypertriglyceridemia in mice. J Biol Chem 288:25350-61 (2013). Read more (PubMed: 23888053) »
  • Cabodevilla AG  et al. Cell survival during complete nutrient deprivation depends on lipid droplet-fueled ß-oxidation of fatty acids. J Biol Chem 288:27777-88 (2013). Read more (PubMed: 23940052) »

See all 2 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Cell lysate - whole cell (Mouse embryonic fibroblast)
Loading amount 25 µg
Specification Mouse embryonic fibroblast
Gel Running Conditions Reduced Denaturing (10-20% tris-tricine)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
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提交于 May 27 2011

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