Alexa Fluor® 488荧光Anti-CPT1A抗体[8F6AE9] (ab171449)
Key features and details
- Alexa Fluor® 488 Mouse monoclonal [8F6AE9] to CPT1A
- Suitable for: ICC/IF, Flow Cyt (Intra)
- Knockout validated
- Reacts with: Human
- Conjugation: Alexa Fluor® 488. Ex: 495nm, Em: 519nm
- Isotype: IgG2b
Related conjugates and formulations
概述
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产品名称
Alexa Fluor® 488荧光Anti-CPT1A抗体[8F6AE9]
参阅全部 CPT1A 一抗 -
描述
Alexa Fluor® 488荧光小鼠单克隆抗体[8F6AE9] to CPT1A -
宿主
Mouse -
偶联物
Alexa Fluor® 488. Ex: 495nm, Em: 519nm -
经测试应用
适用于: ICC/IF, Flow Cyt (Intra)more details -
种属反应性
与反应: Human
预测可用于: Mouse, Rat不与反应: Cow -
免疫原
Recombinant fragment corresponding to Human CPT1A aa 450 to the C-terminus (C terminal).
Database link: P50416 -
阳性对照
- Flow Cyt (Intra): HeLa cells, HAP1-WT cells. ICC/IF: HeLa cells.
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常规说明
Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Product was previously marketed under the MitoSciences sub-brand.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C. Avoid freeze / thaw cycle. Stable for 12 months at -20°C. Store In the Dark. -
存储溶液
Preservative: 0.02% Sodium azide
Constituents: 1% BSA, 98% PBS -
Concentration information loading...
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纯度
Ammonium Sulphate Precipitation -
纯化说明
Purity is near homogeneity as judged by SDS-PAGE. ab171449 was produced in vitro using hybridomas grown in serum-free medium, and then concentrated by ammonium sulfate precipitation. -
克隆
单克隆 -
克隆编号
8F6AE9 -
同种型
IgG2b -
研究领域
相关产品
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Alternative Versions
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Related Products
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab171449于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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ICC/IF |
Use a concentration of 5 µg/ml.
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Flow Cyt (Intra) |
Use a concentration of 5 µg/ml.
ab171465 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody. |
说明 |
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ICC/IF
Use a concentration of 5 µg/ml. |
Flow Cyt (Intra)
Use a concentration of 5 µg/ml. ab171465 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody. |
靶标
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组织特异性
Strong expression in kidney and heart, and lower in liver and skeletal muscle. -
通路
Lipid metabolism; fatty acid beta-oxidation. -
疾病相关
Defects in CPT1A are the cause of carnitine palmitoyltransferase 1A deficiency (CPT1AD) [MIM:255120]; also known as CPT-I deficiency or CPT1A deficiency. CPT1AD is a rare autosomal recessive metabolic disorder of long-chain fatty acid oxidation characterized by severe episodes of hypoketotic hypoglycemia usually occurring after fasting or illness. Onset is in infancy or early childhood. -
序列相似性
Belongs to the carnitine/choline acetyltransferase family. -
细胞定位
Mitochondrion outer membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 1374 Human
- Entrez Gene: 12894 Mouse
- Entrez Gene: 25757 Rat
- Omim: 600528 Human
- SwissProt: P50416 Human
- SwissProt: P97742 Mouse
- SwissProt: P32198 Rat
- Unigene: 503043 Human
see all -
别名
- Carnitine O palmitoyltransferase 1 liver isoform antibody
- Carnitine O palmitoyltransferase I antibody
- Carnitine O palmitoyltransferase I liver isoform antibody
see all
图片
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ab171449 staining CPT1A in HeLa cells. The cells were fixed with 100% methanol (5min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab171449 at 1/1000 dilution (shown in green) and ab190573, Rabbit monoclonal to alpha Tubulin (Alexa Fluor® 647), at 1/250 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue).
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
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Overlay histogram showing HAP1 wildtype (green line) and HAP1-CPT1A knockout cells (red line) stained with ab171449. The cells were fixed with 4% formaldehyde (10 min) and then permeabilized with 0.1% PBS-Triton X-100 for 15 min. The cells were then incubated in 1x PBS / 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (ab171449, 1µg/ml dilution) for 30 min at 22°C.
A rabbit monoclonal IgG isotype control antibody (ab199091) was used at the same concentration and conditions as the primary antibody (HAP1 wildtype - black line, HAP1-CPT1A knockout - grey line). Unlabelled sample was also used as a control (this line is not shown for the purpose of simplicity).
Acquisition of >5,000 events were collected using a 50 mW Blue laser (488nm) and 530/30 bandpass filter. -
Flow cytometric analysis of HeLa cells (4% paraformaldehyde-fixed; methanol-permeablized) labeling CPT1A with ab171449 at 5 µg/mL (green) compared with an isotype control ab170192 at 5 µg/mL (black). Isotype control was labeled with a Goat Anti-mouse Alexa Fluor® 488 secondary antibody prior to signal measurement on the FL-1 channel.
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Immunofluoresecence analysis of HeLa cells (4% paraformaldehyde-fixed, methanol-permeablized) labeling CPT1A with ab171449 at 5µg/ml overnight. 100x magnification.
A) HeLa cells labeled with ab171449 at 5 µg/mL.
B) HeLa cells labeled with Anti-HSP60 (1/3000, ab46798), Secondary antibody used was goat anti-rabbit Dylight-594 (1/1000, ab96897).
C) Merged Image of A and B showing specificity of mitochondrial staining.
实验方案
数据表及文件
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Datasheet download
文献 (11)
ab171449 被引用在 11 文献中.
- Khurana P et al. Distinct Bioenergetic Features of Human Invariant Natural Killer T Cells Enable Retained Functions in Nutrient-Deprived States. Front Immunol 12:700374 (2021). PubMed: 34434191
- Mohammadpour H et al. β2-adrenergic receptor signaling regulates metabolic pathways critical to myeloid-derived suppressor cell function within the TME. Cell Rep 37:109883 (2021). PubMed: 34706232
- Gamradt S et al. Reduced mitochondrial respiration in T cells of patients with major depressive disorder. iScience 24:103312 (2021). PubMed: 34765928
- Thompson EA et al. Metabolic programs define dysfunctional immune responses in severe COVID-19 patients. Cell Rep 34:108863 (2021). PubMed: 33691089
- Kumar B et al. Exosomes-driven lipolysis and bone marrow niche remodeling supports leukemia expansion. Haematologica 106:1484-1488 (2020). PubMed: 33054109