概述

  • 产品名称Anti-CPSF73抗体
    参阅全部 CPSF73 一抗
  • 描述
    兔多克隆抗体to CPSF73
  • 经测试应用适用于: IHC-P, WBmore details
    不适用于: IP
  • 种属反应性
    与反应: Mouse, Human
    预测可用于: Rat, Rabbit, Horse, Guinea pig, Cow, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan
  • 免疫原

    A region between residue 1 and 50 of human CPSF73 (NP_057291.1)

  • 阳性对照
    • Whole cell lysates from HeLa, 293T and mouse NIH3T3 cells.

性能

应用

Our Abpromise guarantee covers the use of ab72294 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P 1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/2000 - 1/10000. Detects a band of approximately 77 kDa (predicted molecular weight: 77 kDa).
  • 应用说明Is unsuitable for IP.
  • 靶标

    • 功能Component of the cleavage and polyadenylation specificity factor (CPSF) complex that play a key role in pre-mRNA 3'-end formation, recognizing the AAUAAA signal sequence and interacting with poly(A) polymerase and other factors to bring about cleavage and poly(A) addition. Has endonuclease activity, and functions as mRNA 3'-end-processing endonuclease. Also involved in the histone 3'-end pre-mRNA processing. U7 snRNP-dependent protein that induces both the 3'-endoribonucleolytic cleavage of histone pre-mRNAs and acts as a 5' to 3' exonuclease for degrading the subsequent downstream cleavage product (DCP) of mature histone mRNAs. Cleavage occurs after the 5'-ACCCA-3' sequence in the histone pre-mRNA leaving a 3'hydroxyl group on the upstream fragment containing the stem loop (SL) and 5' phosphate on the downstream cleavage product (DCP) starting with CU nucleotides. The U7-dependent 5' to 3' exonuclease activity is processive and degrades the DCP RNA substrate even after complete removal of the U7-binding site. Binds to the downstream cleavage product (DCP) of histone pre-mRNAs and the cleaved DCP RNA substrate in a U7 snRNP dependent manner.
    • 序列相似性Belongs to the metallo-beta-lactamase superfamily. RNA-metabolizing metallo-beta-lactamase-like family. CPSF3 subfamily.
    • 翻译后修饰Sumoylated on Lys-462, Lys-465 and Lys-545, preferentially by SUMO3.
    • 细胞定位Nucleus.
    • Information by UniProt
    • 数据库链接
    • 别名
      • Cleavage and polyadenylation specific factor 3 73kDa antibody
      • Cleavage and polyadenylation specificity factor 73 kDa subunit antibody
      • Cleavage and polyadenylation specificity factor subunit 3 antibody
      • CPSF 73 antibody
      • CPSF 73 kDa subunit antibody
      • CPSF antibody
      • CPSF-73 antibody
      • cpsf3 antibody
      • CPSF3_HUMAN antibody
      • CPSF73 antibody
      • mRNA 3''-end-processing endonuclease CPSF-73 antibody
      • YSH1 antibody
      see all

    Anti-CPSF73 antibody 图像

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human prostate carcinoma tissue labelling CPSF73 with ab72294 at 1/1000 (1µg/ml). Detection: DAB.
    • All lanes : Anti-CPSF73 antibody (ab72294) at 0.1 µg/ml

      Lane 1 : HeLa whole cell lysate at 5 µg
      Lane 2 : HeLa whole cell lysate at 15 µg
      Lane 3 : HeLa whole cell lysate at 50 µg
      Lane 4 : 293T whole cell lysate at 50 µg
      Lane 5 : Mouse NIH3T3 whole cell lysate at 50 µg

      Developed using the ECL technique

      Predicted band size : 77 kDa
      Observed band size : 77 kDa


      Exposure time : 3 minutes

    Anti-CPSF73 antibody (ab72294)参考文献

    ab72294 has not yet been referenced specifically in any publications.

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