Anti-CPSF30抗体(ab21865)

概述

  • 产品名称Anti-CPSF30抗体
    参阅全部 CPSF30 一抗
  • 描述
    小鼠多克隆抗体to CPSF30
  • 经测试应用适用于: WBmore details
  • 种属反应性
    与反应: Drosophila melanogaster
  • 免疫原

    Fusion protein:

    ITRNGQECDKGSACPFRHIRGDRTIVCKHWLRGLCKKGDQCEFLHEYDMT KMPECYFYSRFNACHNKECPFLHIDPQSKVKDCPWYKRGFCRHGPHCRHQ

    , corresponding to amino acids 44/143 of Drosophila melanogaster CPSF30.

  • 常规说明

    Produced from outbred CD1 mice

     

    This antibody was raised by a genetic immunization technique. Genetic immunization can be used to generate antibodies by directly delivering antigen-coding DNA into the animal, rather than injecting a protein or peptide (Tang et al. PubMed: 1545867; Chambers and Johnston PubMed: 12910245; Barry and Johnston PubMed: 9234514). The animal`s cells produce the protein, which stimulates the animal`s immune system to produce antibodies against that particular protein. A vector coding for a partial fusion protein was used for genetic immunisation of a mouse and the resulting serum was tested in Western blot against an E.coli lysate containing that partial fusion protein. Genetic immunization offers enormous advantages over the traditional protein-based immunization method. DNA is faster, cheaper and easier to produce and can be produced by standard techniques readily amenable to automation. Furthermore, the antibodies generated by genetic immunization are usually of superior quality with regard to specificity, affinity and recognizing the native protein.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • 存储溶液Constituents: 50% Glycerol
  • 纯度Whole antiserum
  • Primary antibody说明This antibody was raised by a genetic immunization technique. Genetic immunization can be used to generate antibodies by directly delivering antigen-coding DNA into the animal, rather than injecting a protein or peptide (Tang et al. PubMed: 1545867; Chambers and Johnston PubMed: 12910245; Barry and Johnston PubMed: 9234514). The animal`s cells produce the protein, which stimulates the animal`s immune system to produce antibodies against that particular protein. A vector coding for a partial fusion protein was used for genetic immunisation of a mouse and the resulting serum was tested in Western blot against an E.coli lysate containing that partial fusion protein. Genetic immunization offers enormous advantages over the traditional protein-based immunization method. DNA is faster, cheaper and easier to produce and can be produced by standard techniques readily amenable to automation. Furthermore, the antibodies generated by genetic immunization are usually of superior quality with regard to specificity, affinity and recognizing the native protein.
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab21865 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000. Predicted molecular weight: 33 kDa.

This antibody has been tested in Western blot against an E.coli lysate containing the partial recombinant fusion protein used as an immunogen. We have no data on detection of endogenous protein.

靶标

  • 功能Component of the cleavage and polyadenylation specificity factor (CPSF) complex that play a key role in pre-mRNA 3'-end formation, recognizing the AAUAAA signal sequence and interacting with poly(A) polymerase and other factors to bring about cleavage and poly(A) addition. CPSF4 binds RNA polymers with a preference for poly(U).
  • 序列相似性Belongs to the CPSF4/YTH1 family.
    Contains 5 C3H1-type zinc fingers.
    Contains 1 CCHC-type zinc finger.
  • 细胞定位Nucleus.
  • Information by UniProt
  • 别名
    • cleavage and polyadenylation specific factor 4, 30kDa antibody
    • Cleavage and polyadenylation specificity factor 30 kDa subunit antibody
    • Cleavage and polyadenylation specificity factor subunit 4 antibody
    • cleavage polyadenylation specificity factor, 30kD antibody
    • CPSF 30 kDa subunit antibody
    • CPSF30 antibody
    • Cpsf4 antibody
    • CPSF4_HUMAN antibody
    • NAR antibody
    • Neb-1 antibody
    • NEB1 antibody
    • No arches homolog antibody
    • no arches like zinc finger protein antibody
    • NS1 effector domain binding protein 1 antibody
    • NS1 effector domain-binding protein 1 antibody
    • OTTHUMP00000206235 antibody
    • OTTHUMP00000206237 antibody
    • OTTHUMP00000206239 antibody
    see all

Anti-CPSF30 antibody 图像

  • All lanes : Anti-CPSF30 antibody (ab21865) at 1/1000 dilution

    Lane 1 : Total protein extract from E. coli with ~50ng to 100ng of a negative control fusion protein with an irrelevant antigen at 20 ug
    Lane 2 : Total protein extract from E. coli with ~50ng to 500ng of the antigen fusion protein at 20 ug

    Secondary
    Rabbit anti-mouse IgG + IgM, (H+L) horseradish peroxidase conjugated at 1/5000 dilution

    Predicted band size : 33 kDa

Anti-CPSF30 antibody (ab21865)参考文献

ab21865 has not yet been referenced specifically in any publications.

Product Wall

Thank you for contacting us. You may be interested in our flyer regarding Drosophila antibodies:

http://docs.abcam.com/pdf/general/Drosophila_Melanogaster_Antibodies_with_cross_activity.pdf

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