概述

  • 产品名称
  • 描述
    兔多克隆抗体to CPSF1
  • 经测试应用
    适用于: ICC/IF, IHC-Pmore details
  • 种属反应性
    与反应: Mouse, Human
    预测可用于: Rat, Horse, Guinea pig, Cow, Dog, Pig, Rhesus monkey, Gorilla, Chinese hamster, Orangutan, Elephant
  • 免疫原

    Synthetic peptide, corresponding to a region within the amino acids 1392-1442 of Human CPSF1, NP_037423.1

  • 阳性对照
    • Human anaplastic thyroid carcinoma, bladder cell carcinoma, breast carcinoma, colon carcinoma, linitis plastica stomach cancer, ovarian carcinoma, prostate carcinoma, skin basal cell carcinoma, small cell lung cancer, stomach adenocarcinoma, testicular seminoma. Mouse colon carcinoma CT26, renal cell carcinoma, teratoma.

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液
    Preservative: 0.09% Sodium Azide
    Constituents: 0.1% BSA, Tris buffered saline
  • Concentration information loading...
  • 纯度
    Immunogen affinity purified
  • 纯化说明
    Antibody was affinity purified using an epitope specific to CPSF160 immobilized on solid support.
  • 克隆
    多克隆
  • 同种型
    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab84743 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF
IHC-P
  • 应用说明
    ICC/IF: 1/100.
    IHC-P: 1/100 - 1/500. Epitope exposure is recommended. Epitope exposure with citrate will enhance staining.

    Likely to work with frozen sections.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 相关性
      CPSF1 (Cleavage and polyadenylation specificity factor subunit 1) is a component of the cleavage and polyadenylation specificity factor (CPSF) complex. It plays a key role in pre-mRNA 3'-end formation, recognizing the AAUAAA signal sequence and interacting with poly(A) polymerase and other factors to bring about cleavage and poly(A) addition. This subunit is involved in the RNA recognition step of the polyadenylation reaction.
    • 细胞定位
      Nucleus, nucleoplasm.
    • 数据库链接
    • 别名
      • Cleavage and polyadenylation specificity factor 160 kDa subunit antibody
      • Cleavage and polyadenylation specificity factor subunit 1 antibody
      • CPSF 160 kDa subunit antibody
      • CPSF160 antibody
      • HSU37012 antibody
      • P/cl.18 antibody
      see all

    图片

    • Detection of Human CPSF1 by IHC-IF.
      Sample: FFPE section of Human breast carcinoma.
      Antibody: ab84743 used at a dilution of 1/100.
      Detection: Red-fluorescent goat anti-rabbit IgG highly cross-adsorbed Antibody Hilyte PlusTM 555 used at a dilution of 1/100.
    • Detection of Human CPSF1 by Immunohistochemistry.
      Sample: FFPE section of Human breast carcinoma.
      Antibody: ab84743 used at a dilution of 1/100.
      Detection: DAB staining.
    • Detection of Human CPSF1 by Immunohistochemistry.
      Sample: FFPE section of Human prostate carcinoma.
      Antibody: ab84743 used at a dilution of 1/500.
      Detection: DAB staining.
    • Detection of Mouse CPSF1 by Immunohistochemistry.
      Sample: FFPE section of Mouse teratoma.
      Antibody: ab84743 used at a dilution of 1/100.
      Detection: DAB staining.

    文献

    ab84743 has not yet been referenced specifically in any publications.

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