概述

  • 产品名称
  • 描述
    兔多克隆抗体to CPSF1
  • 经测试应用
    适用于: WB, IP, IHC-Pmore details
  • 种属反应性
    与反应: Mouse, Human
    预测可用于: Rat, Horse, Guinea pig, Cow, Dog, Pig, Rhesus monkey, Gorilla, Chinese hamster, Orangutan, Elephant
  • 免疫原

    Synthetic peptide corresponding to a region between residue 1392 and 1442 of Human CPSF1 (NP_037423.1).

  • 阳性对照
    • HeLa, 293T and NIH3T3 whole cell lysates.

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液
    Preservative: 0.09% Sodium Azide
    Constituents: 0.1% BSA, Tris buffered saline
  • Concentration information loading...
  • 纯度
    Immunogen affinity purified
  • 克隆
    多克隆
  • 同种型
    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab81552 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/2000 - 1/10000. Predicted molecular weight: 161 kDa.
IP Use at 2-5 µg/mg of lysate.
IHC-P Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

靶标

  • 相关性
    CPSF1 (Cleavage and polyadenylation specificity factor subunit 1) is a component of the cleavage and polyadenylation specificity factor (CPSF) complex. It plays a key role in pre-mRNA 3'-end formation, recognizing the AAUAAA signal sequence and interacting with poly(A) polymerase and other factors to bring about cleavage and poly(A) addition. This subunit is involved in the RNA recognition step of the polyadenylation reaction.
  • 细胞定位
    Nucleus, nucleoplasm.
  • 数据库链接
  • 别名
    • Cleavage and polyadenylation specificity factor 160 kDa subunit antibody
    • Cleavage and polyadenylation specificity factor subunit 1 antibody
    • CPSF 160 kDa subunit antibody
    • CPSF160 antibody
    • HSU37012 antibody
    • P/cl.18 antibody
    see all

图片

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human ovarian carcinoma (left) and mouse renal cell carcinoma (right) tissues labelling CPSF1 with ab81552 at 1/200 (1µg/ml). Detection: DAB.
  • All lanes : Anti-CPSF1 antibody (ab81552) at 0.04 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg
    Lane 4 : 293T whole cell lysate at 50 µg
    Lane 5 : NIH3T3 whole cell lysate at 50 µg

    Developed using the ECL technique

    Predicted band size : 161 kDa
    Observed band size : 171 kDa (why is the actual band size different from the predicted?)


    Exposure time : 10 seconds
  • HeLa cell lysate (1mg) was immunoprecipitated using ab81552 at 3µg/mg lysate (lane 1) or IgG control (lane 2). 20% of the immunoprecipitate was analysed by Western blot using ab81552 at 1µg/ml. Bands were detected by chemiluminescence with an exposure time of 10 seconds.
  • IHC image of ab81552 staining in Human Breast cancer formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab81552, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

文献

ab81552 has not yet been referenced specifically in any publications.

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