概述

  • 产品名称Anti-CPSF1抗体
    参阅全部 CPSF1 一抗
  • 描述
    兔多克隆抗体to CPSF1
  • 经测试应用适用于: WB, ELISAmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    A region within synthetic peptide: GCYDMWTVIA PVRKEEEDNP KGEGTEQEPS TTPEADDDGR RHGFLILSRE, corresponding to amino acids 531-580 of Human CPSF1

  • 阳性对照
    • 293T cell lysate.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液Preservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab62598 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 0.5 µg/ml. Detects a band of approximately 144 kDa (predicted molecular weight: 161 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

ELISA titre using peptide based assay: 1/62500.

靶标

  • 相关性CPSF1 (Cleavage and polyadenylation specificity factor subunit 1) is a component of the cleavage and polyadenylation specificity factor (CPSF) complex. It plays a key role in pre-mRNA 3'-end formation, recognizing the AAUAAA signal sequence and interacting with poly(A) polymerase and other factors to bring about cleavage and poly(A) addition. This subunit is involved in the RNA recognition step of the polyadenylation reaction.
  • 细胞定位Nucleus, nucleoplasm.
  • 数据库链接
  • 别名
    • Cleavage and polyadenylation specificity factor 160 kDa subunit antibody
    • Cleavage and polyadenylation specificity factor subunit 1 antibody
    • CPSF 160 kDa subunit antibody
    • CPSF160 antibody
    • HSU37012 antibody
    • P/cl.18 antibody
    see all

Anti-CPSF1 antibody 图像

  • Anti-CPSF1 antibody (ab62598) at 0.5 µg/ml + 293T cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 161 kDa
    Observed band size : 144 kDa (why is the actual band size different from the predicted?)

Anti-CPSF1 antibody (ab62598)参考文献

ab62598 has not yet been referenced specifically in any publications.

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