概述

  • 产品名称Anti-CPNE9抗体
  • 描述
    兔多克隆抗体to CPNE9
  • 经测试应用适用于: WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Pig
  • 免疫原

    Synthetic peptide corresponding to a region within the internal amino acids 216-265 (DRTVKIDVYD WDRDGSHDFI GEFTTSYREL SKAQNQFTVY EVLNPRKKCK) of Human CPNE9 (NP_705899).

  • 阳性对照
    • 721_B cell lysate.

性能

应用

Our Abpromise guarantee covers the use of ab87686 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Detects a band of approximately 60 kDa (predicted molecular weight: 56 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

靶标

Anti-CPNE9 antibody 图像

  • Anti-CPNE9 antibody (ab87686) at 1 µg/ml (in 5% skim milk / PBS buffer) + 721_B cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 56 kDa
    Observed band size : 60 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 20 kDa,33 kDa. We are unsure as to the identity of these extra bands.

Anti-CPNE9 antibody (ab87686)参考文献

ab87686 has not yet been referenced specifically in any publications.

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