产品名称 Anti-CPNE1抗体 参阅全部 CPNE1 一抗
WB, ELISA, IHC-P more details
Mouse, Rat, Sheep, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Pig
Synthetic peptide: ETVQKLRFGI YDIDNKTPEL RDDDFLGGAE CSLGQIVSSQ VLTLPLMLKP, corresponding to amino acids 74-123 of Human CPNE1
HepG2 cell lysate. Human intestine.
存放说明 Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
存储溶液 Preservative: None
Constituents: 2% Sucrose, PBS
Concentration information loading...
纯度 Protein A purified
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 5 µg/ml. Detects a band of approximately 57 kDa (predicted molecular weight: 59 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
Use at an assay dependent concentration.
Titre using peptide based assay: 1/312500.
Use a concentration of 4 - 8 µg/ml.
功能 May function in membrane trafficking. Exhibits calcium-dependent phospholipid binding properties.
序列相似性 Belongs to the copine family. Contains 2 C2 domains. Contains 1 VWFA domain.
Information by UniProt
Copine 1 antibody
Copine I antibody
Anti-CPNE1 antibody 图像
Western blot - CPNE1 antibody (ab66898)
All lanes : Anti-CPNE1 antibody (ab66898) at 5 µg/ml (in 5% skim milk / PBS buffer) Lane 1 : Molecular weight markers Lane 2 : HepG2 cell lysate at 10 µg Secondary HRP conjugated anti-Rabbit IgG at 1/50000 dilution Predicted band size : 59 kDa Observed band size : 57 kDa ( why is the actual band size different from the predicted?) Additional bands at : 25 kDa. We are unsure as to the identity of these extra bands. Gel concentration 12%.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - CPNE1 antibody (ab66898)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human intestine tissue labelling CPNE1 with ab66898 at 4-8µg/ml. Arrows indicate positively labelled epithelial cells of the intestinal villus. Magnification: 400X.
Anti-CPNE1 antibody (ab66898)参考文献
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"