重组
RabMAb

Anti-Cpn10抗体[EPR4476] (ab108600)

概述

  • 产品名称
    Anti-Cpn10抗体[EPR4476]
    参阅全部 Cpn10 一抗
  • 描述
    兔单克隆抗体[EPR4476] to Cpn10
  • 经测试应用
    适用于: WB, IP, IHC-P, ICCmore details
    不适用于: Flow Cyt
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide corresponding to residues in Human Cpn10.

  • 阳性对照
    • WB: A431, Neuro-2a, A549, and MCF7 cell lysates IHC-P: Human colon and kidney tissues ICC/IF: HeLa cells
  • 常规说明

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. 

    For details on our patents, please refer to www.abcam.com/RabMAbPatents

    This product is a recombinant rabbit monoclonal antibody.

性能

应用

Our Abpromise guarantee covers the use of ab108600 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/10000 - 1/50000. Detects a band of approximately 11 kDa (predicted molecular weight: 11 kDa).
IP 1/10 - 1/100.
IHC-P 1/250 - 1/500.
ICC 1/100 - 1/250.
  • 应用说明
    Is unsuitable for Flow Cyt.
  • 靶标

    • 功能
      Eukaryotic CPN10 homolog which is essential for mitochondrial protein biogenesis, together with CPN60. Binds to CPN60 in the presence of Mg-ATP and suppresses the ATPase activity of the latter.
    • 序列相似性
      Belongs to the GroES chaperonin family.
    • 细胞定位
      Mitochondrion matrix.
    • Information by UniProt
    • 数据库链接
    • 别名
      • 10 kDa chaperonin antibody
      • 10 kDa heat shock protein mitochondrial antibody
      • 10 kDa heat shock protein, mitochondrial antibody
      • CH10_HUMAN antibody
      • Chaperonin 10 antibody
      • Chaperonin 10 homolog antibody
      • CPN10 antibody
      • cpn10 homolog antibody
      • Early pregnancy factor antibody
      • Early-pregnancy factor antibody
      • EPF antibody
      • GROES antibody
      • GroES homolog antibody
      • Heat shock 10kD protein 1 chaperonin 10 antibody
      • Heat shock 10kDa protein 1 antibody
      • Heat shock 10kDa protein 1 chaperonin 10 antibody
      • Heat shock protein family E (Hsp10) member antibody
      • Heat-shock 10-kD protein antibody
      • Hsp10 antibody
      • Hspe1 antibody
      see all

    Anti-Cpn10 antibody [EPR4476] 图像

    • All lanes : Anti-Cpn10 antibody [EPR4476] (ab108600) at 1/10000 dilution

      Lane 1 : A431 cell lysate
      Lane 2 : Neuro-2a cell lysate
      Lane 3 : A549 cell lysate
      Lane 4 : MCF7 cell lysate

      Lysates/proteins at 10 µg per lane.


      Predicted band size : 11 kDa
      Observed band size : 11 kDa
    • Immunohistochemical staining of Cpn10 in paraffin embedded Human colon tissue, using ab108600 at a dilution of 1/250.
    • Immunohistochemical staining of Cpn10 in paraffin embedded Human kidney tissue, using ab108600 at a dilution of 1/250.
    • Immunofluorescent staining of Cpn10 in HeLa cells, using ab108600 at a dilution of 1/100.
    • ab108600 showing positive staining in Normal uterus tissue.

    • ab108600 showing positive staining in Ovarian carcinoma tissue.

    • ab108600 showing positive staining in Normal breast tissue.

    • ab108600 showing positive staining in Colonic adenocarcinoma tissue.

    • ab108600 showing positive staining in Normal stomach tissue.

    Anti-Cpn10 antibody [EPR4476] (ab108600)参考文献

    This product has been referenced in:
    • Sakellariou GK  et al. Neuron-specific expression of CuZnSOD prevents the loss of muscle mass and function that occurs in homozygous CuZnSOD-knockout mice. FASEB J 28:1666-81 (2014). WB ; Mouse . Read more (PubMed: 24378874) »
    • Gispert S  et al. Loss of mitochondrial peptidase Clpp leads to infertility, hearing loss plus growth retardation via accumulation of CLPX, mtDNA and inflammatory factors. Hum Mol Genet N/A:N/A (2013). Read more (PubMed: 23851121) »

    See all 2 Publications for this product

    Product Wall

    Application
    Western blot
    Sample
    Rat Tissue lysate - whole (Skeletal muscle)
    Gel Running Conditions
    Reduced Denaturing (15%)
    Loading amount
    20 µg
    Specification
    Skeletal muscle
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
    Username

    Abcam user community

    Verified customer

    提交于 Feb 04 2016

    Application
    Immunoprecipitation
    Immuno-precipitation step
    Protein A
    Sample
    Human Cell lysate - whole cell (HEK293 cells)
    Specification
    HEK293 cells
    Total protein in input
    250 µg
    Username

    Abcam user community

    Verified customer

    提交于 Sep 29 2014

    Application
    Western blot
    Loading amount
    50 µg
    Gel Running Conditions
    Reduced Denaturing (15% gel)
    Sample
    Mouse Tissue lysate - whole (Skeletal muscle)
    Specification
    Skeletal muscle
    Blocking step
    Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
    Username

    Abcam user community

    Verified customer

    提交于 Jan 09 2014

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (Jurkat)
    Loading amount
    10 µg
    Specification
    Jurkat
    Gel Running Conditions
    Reduced Denaturing
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 4% · Temperature: 25°C
    Username

    Abcam user community

    Verified customer

    提交于 May 02 2012

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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