Anti-CPI17 alpha (phospho T38)抗体(ab52174)

概述

  • 产品名称Anti-CPI17 alpha (phospho T38)抗体
    参阅全部 CPI17 alpha 一抗
  • 描述
    兔多克隆抗体to CPI17 alpha (phospho T38)
  • 特异性ab52174 detects endogenous levels of CPI17a only when phosphorylated at threonine 38.
  • 经测试应用适用于: IHC-P, ELISA, WBmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    Synthetic phosphopeptide derived from human CPI17a around the phosphorylation site of threonine 38 (R-V-TP-V-K).

  • 阳性对照
    • Human breast carcinoma tissue.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • 存储溶液Preservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 纯化说明ab52174 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab52174 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P Use at an assay dependent concentration.
ELISA 1/20000.
WB 1/1000. Detects a band of approximately 17 kDa (predicted molecular weight: 17 kDa).

靶标

  • 功能Inhibitor of PPP1CA. Has over 1000-fold higher inhibitory activity when phosphorylated, creating a molecular switch for regulating the phosphorylation status of PPP1CA substrates and smooth muscle contraction.
  • 组织特异性Isoform 1 is detected in aorta and testis. Isoform 2 is detected in aorta.
  • 序列相似性Belongs to the PP1 inhibitor family.
  • 细胞定位Cytoplasm.
  • Information by UniProt
  • 数据库链接
  • 别名
    • 17 kDa PKC-potentiated inhibitory protein of PP1 antibody
    • 17-KDa protein antibody
    • 17kDa PKC potentiated inhibitory protein of PP1 antibody
    • CPI 17 alpha antibody
    • CPI 17 antibody
    • CPI 17alpha antibody
    • CPI-17 antibody
    • CPI17 antibody
    • PKC potentiated inhibitory protein of PP1 antibody
    • PKC potentiated inhibitory protein of PP1, 17-KD antibody
    • PP14A_HUMAN antibody
    • PPP1INL antibody
    • Ppp1r14a antibody
    • Protein kinase C-potentiated inhibitor of protein phosphatase 1, 17-KD antibody
    • Protein kinase C-potentiated inhibitor protein of 17 kDa antibody
    • Protein phosphatase 1 antibody
    • Protein phosphatase 1 regulatory (inhibitor) subunit 14A antibody
    • Protein phosphatase 1 regulatory subunit 14A antibody
    • Regulatory subunit 14A antibody
    see all

Anti-CPI17 alpha (phospho T38) antibody 图像

  • ab52174 at 1/50 dilution staining CPI17a in human breast carcinoma by Immunohistochemistry, paraffin embedded tissue, in the presence (right image) and absence (left image) of the immunising peptide.


  • Predicted band size : 17 kDa

Anti-CPI17 alpha (phospho T38) antibody (ab52174)参考文献

This product has been referenced in:
  • Kang S  et al. Dysfunction of vascular smooth muscle and vascular remodeling by simvastatin. Toxicol Sci 138:446-556 (2014). Rat . Read more (PubMed: 24449418) »

See 1 Publication for this product

Product Wall

Thank you for contacting us.

We have not specifically tested ab52147 on C2C12 cells. However, we do have customer feedback regarding the use of this antibody to detect the phosphorylated protein in treated myometrial smooth muscle cells. Read More

Thank you for contacting us.
This antibody has been validated for Western blot using RAW264.7 cell lysate. It has not been tested against other mouse cell lines, such as C2C12.


I hope this information is helpful to you. Please do ...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (Myometrial smooth muscle cells)
Loading amount 15 µg
Specification Myometrial smooth muscle cells
Treatment 1 or 10 nM oxytocin for 1 minute, and 1µM phorbol 12-myristate 13-acetate for 10 minutes
Gel Running Conditions Reduced Denaturing (13% tris-glycine SDS-PAGE gel)
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Username

Abcam user community

Verified customer

提交于 Apr 10 2009

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"