概述

  • 产品名称Anti-CPEB3抗体
  • 描述
    兔多克隆抗体to CPEB3
  • 经测试应用适用于: IHC-Fr, IHC-FoFr, WB, ICC/IFmore details
  • 种属反应性
    与反应: Mouse, Rat
    预测可用于: Human
  • 免疫原

    Synthetic peptide derived from residues 300 - 400 of Mouse CPEB3.

  • 阳性对照
    • This antibody gave a positive signal when tested against Mouse Brain Tissue. This antibody gave a positive signal in the following cell types: SKNSH.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液Preservative: 0.02% Sodium Azide
    Constituents: 1% BSA, PBS, pH 7.4
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

相关产品

应用

Our Abpromise guarantee covers the use of ab10883 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-Fr Use at an assay dependent concentration.
IHC-FoFr 1/100.
WB 1/500 - 1/1000. Detects a band of approximately 75 kDa (predicted molecular weight: 75 kDa).
ICC/IF Use a concentration of 5 µg/ml.

靶标

Anti-CPEB3 antibody 图像

  • ab10883 staining CPEB3 in mouse brain tissue section by Immunohistochemistry (PFA perfusion fixed frozen sections). Tissue from 4% PFA perfused animals underwent overnight fixation in 4% paraformaldehyde, cryoprotected in 30% sucrose and cut using cryostat.The primary antibody was diluted, 1/100 (PBS + 0.3% Triton X100) and incubated with sample for 18 hours at 20°C. An Alexa Fluor®488 conjugated goat polyclonal to rabbit IgG at 1/1000 dilution, was used as secondary.

    See Abreview

  • ICC/IF image of ab10883 stained SKNSH cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab10883 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Lane 1 : Anti-CPEB3 antibody (ab10883) at 1/500 dilution
    Lane 2 : Anti-CPEB3 antibody (ab10883) at 1/1000 dilution

    Lane 1 : Mouse Brain lysate
    Lane 2 : Mouse Brain lysate

    Lysates/proteins at 5 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab7090) at 1/5000 dilution

    Predicted band size : 75 kDa


    Exposure time : 3 minutes

    We think that the top band here is the correct band, as it is the same size as the most intense band on the previous blot. The banding pattern is the same on both blots and the lower bands may be clevage products as there is no evidence of CPEB3 isoforms in the literature.

Anti-CPEB3 antibody (ab10883)参考文献

This product has been referenced in:
  • Pedraza N  et al. KIS, a kinase associated with microtubule regulators, enhances translation of AMPA receptors and stimulates dendritic spine remodeling. J Neurosci 34:13988-97 (2014). WB, IP ; Mouse . Read more (PubMed: 25319695) »
  • La Via L  et al. Modulation of dendritic AMPA receptor mRNA trafficking by RNA splicing and editing. Nucleic Acids Res 41:617-31 (2013). ICC/IF ; Rat . Read more (PubMed: 23166306) »

See all 2 Publications for this product

Product Wall

Abreviews
Abcam has not validated the combination of species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (Hela cells)
Gel Running Conditions Reduced Denaturing (5-20% gradient)
Loading amount 20 µg
Specification Hela cells
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Username

Abcam user community

Verified customer

提交于 Dec 21 2015

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Sample Mouse Tissue sections (Brain sections)
Specification Brain sections
Fixative Paraformaldehyde
Permeabilization Yes - Triton X100
Blocking step Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 22°C
Username

Mr. Amir Djourabchi

Verified customer

提交于 Apr 13 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample Mouse Tissue sections (Brain sections)
Specification Brain sections
Fixative Paraformaldehyde
Antigen retrieval step None
Permeabilization No
Username

Dr. Sophie Pezet

Verified customer

提交于 Jun 17 2009

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"