Anti-CPEB2抗体(ab51069)

概述

  • 产品名称Anti-CPEB2抗体
    参阅全部 CPEB2 一抗
  • 描述
    兔多克隆抗体to CPEB2
  • 经测试应用适用于: WB, ELISA, IHC-Frmore details
  • 种属反应性
    与反应: Mouse, Human
    预测可用于: Rat, Pig
  • 免疫原

    A region within synthetic peptide: SNTLLPLQVR SSLQLPAWGS DSLQDSWCTA AGTSRIDQDR SRMYDSLNMH, corresponding to amino acids 204-253 of Human CPEB2

  • 阳性对照
    • Human Ovary Tumor tissue lysate

性能

相关产品

应用

Our Abpromise guarantee covers the use of ab51069 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 0.25 - 1 µg/ml. Detects a band of approximately 40 kDa (predicted molecular weight: 66 kDa).

Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

ELISA 1/312500.
IHC-Fr 1/250.

靶标

  • 相关性CPEB2, (Cytoplasmic polyadenylation element-binding protein 2), belongs to the RRM CPEB family and contains 2 RRM (RNA recognition motif) domains. It may bind poly(U) RNA oligomers. It may play a role in translational regulation of stored mRNAs in transcriptionally inactive haploid spermatids. There are 7 named isoforms [FASTA] produced by alternative splicing.
  • 细胞定位Cytoplasmic
  • 数据库链接
  • 别名
    • CPE binding protein 2 antibody
    • CPE BP2 antibody
    • CPEB 2 antibody
    • CPEBP2 antibody
    • Cytoplasmic polyadenylation element binding protein 2 antibody
    • hCPEB 2 antibody
    • hCPEB2 antibody
    see all

Anti-CPEB2 antibody 图像

  • Anti-CPEB2 antibody (ab51069) at 1 µg/ml + Human Ovary Tumor tissue lysate at 25 µg

    Secondary
    HRP conjugated Goat anti-Rabbit IgG at 1/10000 dilution

    Predicted band size : 66 kDa
  • ab51069 staining CPEB2 in mouse brain (Wild Type) sections. After perfusion in 4% PFA and PBS 1X, animal underwent 24h fixation in 4% PFA following cryoprotection in 30% sucrose. Brain was cut in 40µm thick sections using cryostat. Sections were blocked for 2h by using 10% NGS, 0.4% Triton X in PBS (pH 7.4). Primary Ab was diluted 1:250 (5% NGS, 0.2% Triton X) and incubated overnight at 4°C. Sections were incubated afterwards with secondary Ab Alexa Fluor 594 goat anti rabbit (1:500) for 1 1/2h. Hoechst was used for nucleus staining (1:100 in A. dest.) for 10 min. Region: Cortex

Anti-CPEB2 antibody (ab51069)参考文献

ab51069 has not yet been referenced specifically in any publications.

Product Wall

I have had a reply from the lab concerning ab51069. At the time they did the WB that we show on the datasheet with Jurkat cell lysate, the size of CPEB2 from their records was ˜40kD. They did not verify this band with the blocking peptide. Since then, ...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Sample Mouse Tissue sections (Brain sections)
Specification Brain sections
Fixative Paraformaldehyde
Permeabilization Yes - Triton X100
Blocking step Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 22°C
Username

Mr. Amir Djourabchi

Verified customer

提交于 Apr 20 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"