概述

  • 产品名称
  • 描述
    兔多克隆抗体to CP110
  • 经测试应用
    适用于: WB, IPmore details
  • 种属反应性
    与反应: Human
    预测可用于: Rabbit, Horse, Guinea pig, Cow, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Elephant
  • 免疫原

    Synthetic peptide corresponding to a region within amino acids 962-1012 of Human CP110 (CAH18111.1).

  • 阳性对照
    • HeLa and 293T whole cell lysates

性能

应用

Our Abpromise guarantee covers the use of ab99338 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/2000 - 1/10000. Predicted molecular weight: 113 kDa.
IP Use at 2-5 µg/mg of lysate.

靶标

  • 功能
    Necessary for centrosome duplication at different stages of procentriole formation. Collaborates with CEP97, being involved in the suppression of a cilia assembly program. Required for correct spindle formation and has a role in regulating cytokinesis and genome stability via cooperation with CALM1 and CETN2.
  • 组织特异性
    Highly expressed in testis. Detected at intermediate levels in spleen, thymus, prostate, small intestine, colon and peripheral blood leukocytes.
  • 翻译后修饰
    Phosphorylated by CDKs.
    Ubiquitinated by the SCF(Cyclin F) during G2 phase, leading to its degradation by the proteasome and preventing centrosome reduplication.
  • 细胞定位
    Cytoplasm > cytoskeleton > centrosome > centriole. Recruited early and then associates with the growing distal tips.
  • Information by UniProt
  • 数据库链接
  • 别名
    • 110 kDa centrosomal protein antibody
    • CCP110 antibody
    • Centriolar coiled coil protein 110kDa antibody
    • Centrosomal protein 110 kDa antibody
    • Centrosomal protein CP110 antibody
    • Centrosomal protein of 110 kDa antibody
    • Cep110 antibody
    • CP110 antibody
    • CP110_HUMAN antibody
    • DKFZp781G1416 antibody
    • FAN antibody
    • KIAA0419 antibody
    see all

图片

  • All lanes : Anti-CP110 antibody (ab99338) at 0.04 µg/ml

    Lane 1 : Hela whole cell lysate at 50 µg
    Lane 2 : Hela whole cell lysate at 15 µg
    Lane 3 : Hela whole cell lysate at 5 µg
    Lane 4 : 293T whole cell lysate at 50 µg

    Developed using the ECL technique

    Predicted band size : 113 kDa


    Exposure time : 30 seconds
  • Detection of Human CP110 by Immunoprecipitation (IP) from HeLa whole cell lysate (1 mg for IP, 20% of IP loaded).
    1. IP with a CP110 antibody which recognizes an upstream epitope
    2. IP with ab99338 at 3 µg/mg lysate
    3. Control IgG IP
    Subsequent WB detection was performed using ab99338 at 1 µg/ml.

文献

This product has been referenced in:
  • Lai Y  et al. Inflammation-mediated upregulation of centrosomal protein 110, a negative modulator of ciliogenesis, in patients with chronic rhinosinusitis. J Allergy Clin Immunol 128:1207-1215.e1 (2011). Read more (PubMed: 21982113) »

See 1 Publication for this product

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