概述

  • 产品名称Anti-CP110抗体
    参阅全部 CP110 一抗
  • 描述
    兔多克隆抗体to CP110
  • 经测试应用适用于: WB, IPmore details
  • 种属反应性
    与反应: Human
    预测可用于: Pig, Chimpanzee, Ferret, Rhesus monkey, Gorilla
  • 免疫原

    Synthetic peptide, corresponding to a region within amino acids within residues 575-625 of Human CP110 (CAH18111.1).

  • 阳性对照
    • HeLa and 293T whole cell lysates

性能

相关产品

应用

Our Abpromise guarantee covers the use of ab99337 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/2000 - 1/10000. Predicted molecular weight: 113 kDa.
IP Use at 2-5 µg/mg of lysate.

靶标

  • 功能Necessary for centrosome duplication at different stages of procentriole formation. Collaborates with CEP97, being involved in the suppression of a cilia assembly program. Required for correct spindle formation and has a role in regulating cytokinesis and genome stability via cooperation with CALM1 and CETN2.
  • 组织特异性Highly expressed in testis. Detected at intermediate levels in spleen, thymus, prostate, small intestine, colon and peripheral blood leukocytes.
  • 翻译后修饰Phosphorylated by CDKs.
    Ubiquitinated by the SCF(Cyclin F) during G2 phase, leading to its degradation by the proteasome and preventing centrosome reduplication.
  • 细胞定位Cytoplasm > cytoskeleton > centrosome > centriole. Recruited early and then associates with the growing distal tips.
  • Information by UniProt
  • 数据库链接
  • 别名
    • 110 kDa centrosomal protein antibody
    • CCP110 antibody
    • Centriolar coiled coil protein 110kDa antibody
    • Centrosomal protein 110 kDa antibody
    • Centrosomal protein CP110 antibody
    • Centrosomal protein of 110 kDa antibody
    • Cep110 antibody
    • CP110 antibody
    • CP110_HUMAN antibody
    • DKFZp781G1416 antibody
    • FAN antibody
    • KIAA0419 antibody
    see all

Anti-CP110 antibody 图像

  • All lanes : Anti-CP110 antibody (ab99337) at 0.04 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg
    Lane 4 : 293T whole cell lysate at 50 µg

    Developed using the ECL technique

    Predicted band size : 113 kDa


    Exposure time : 3 minutes
  • ab99337 at 1µg/ml detecting CP110 in HeLa whole cell lysate by WB following IP.

    Lane 1: ab99337 IP at 3µg/mg of lysate
    Lane 2: IP with an antibody which recognizes an downstream epitope of CP110
    Lane 3: control IgG.

    In each case, 1 mg of lysate was used for IP and 20% of the IP was loaded.
    Detection: Chemiluminescence with an exposure time of 30 seconds.

Anti-CP110 antibody (ab99337)参考文献

This product has been referenced in:
  • Hori A  et al. Centriolar satellite- and hMsd1/SSX2IP-dependent microtubule anchoring is critical for centriole assembly. Mol Biol Cell 26:2005-19 (2015). ICC/IF ; Human . Read more (PubMed: 25833712) »

See 1 Publication for this product

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Tested and Guaranted Applications:

ab11257 - WB, ICC/IF

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