重组Anti-COX5B抗体[EPR14439(B)] (ab180136)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR14439(B)] to COX5B
- Suitable for: Flow Cyt (Intra), IHC-P, WB, ICC/IF, IP
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-COX5B抗体[EPR14439(B)]
参阅全部 COX5B 一抗 -
描述
兔单克隆抗体[EPR14439(B)] to COX5B -
宿主
Rabbit -
经测试应用
适用于: Flow Cyt (Intra), IHC-P, WB, ICC/IF, IPmore details -
种属反应性
与反应: Human
预测可用于: Mouse, Rat -
免疫原
Synthetic peptide within Human COX5B aa 1-100. The exact sequence is proprietary.
Database link: P10606 -
阳性对照
- Human fetal heart lysate; HeLa and HepG2 cells; Human kidney tissue; U87-MG, HepG2 and HeLa cell lysates.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR14439(B) -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab180136于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
Flow Cyt (Intra) |
1/10.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
|
IHC-P |
1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
|
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WB | (1) |
1/10000 - 1/50000. Predicted molecular weight: 14 kDa.
|
ICC/IF |
1/1000.
|
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IP |
1/70.
|
说明 |
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Flow Cyt (Intra)
1/10. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
IHC-P
1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
WB
1/10000 - 1/50000. Predicted molecular weight: 14 kDa. |
ICC/IF
1/1000. |
IP
1/70. |
靶标
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功能
This protein is one of the nuclear-coded polypeptide chains of cytochrome c oxidase, the terminal oxidase in mitochondrial electron transport. -
序列相似性
Belongs to the cytochrome c oxidase subunit 5B family. -
细胞定位
Mitochondrion inner membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 1329 Human
- Entrez Gene: 12859 Mouse
- Entrez Gene: 94194 Rat
- Omim: 123866 Human
- SwissProt: P10606 Human
- SwissProt: P19536 Mouse
- SwissProt: P12075 Rat
- Unigene: 1342 Human
see all -
别名
- COX 5B antibody
- COX5B antibody
- COX5B_HUMAN antibody
see all
图片
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All lanes : Anti-COX5B antibody [EPR14439(B)] (ab180136) at 1/50000 dilution
Lane 1 : Human fetal heart lysate
Lane 2 : HepG2 cell lysate
Lane 3 : HeLa cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 14 kDa -
Immunofluorescence analysis of HepG2 cells labeling COX5B using ab180136 at 1/1000 dilution. A Goat anti rabbit IgG (Dylight 555) at 1/250 dilution was used as secondary antibody. Cells were fixed with 4% paraformaldehyde. Counterstain: DAPI.
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Immunohistochemical analysis of paraffin-embedded human kidney tissue labeling COX5B using ab180136 at 1/500 dilution. A Goat anti rabbit IgG (HRP) prediluted was used as secondary. Counterstain: Hematoxylin.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunoprecipitation analysis of Human fetal heart lysate labeling COX5B using ab180136 at 1/70 dilution. A Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 was used as secondary antibody.
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Anti-COX5B antibody [EPR14439(B)] (ab180136) at 1/10000 dilution + U87-MG cell lysate at 20 µg
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 14 kDa -
Intracellular Flow Cytometry analysis of HeLa cells labeling COX5B using ab180136 at 1/10 dilution. A Goat anti rabbit IgG (FITC) at 1/75 dilution was used as secondary antibody. Cells were fixed with 2% paraformaldehyde. Isotype control: Rabbit IgG.
实验方案
数据表及文件
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SDS download
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Datasheet download
Certificate of Compliance
文献 (6)
ab180136 被引用在 6 文献中.
- Chu YD et al. COX5B-Mediated Bioenergetic Alterations Modulate Cell Growth and Anticancer Drug Susceptibility by Orchestrating Claudin-2 Expression in Colorectal Cancers. Biomedicines 10:N/A (2021). PubMed: 35052740
- Chu YD et al. COX5B-Mediated Bioenergetic Alteration Regulates Tumor Growth and Migration by Modulating AMPK-UHMK1-ERK Cascade in Hepatoma. Cancers (Basel) 12:N/A (2020). PubMed: 32580279
- Li Y et al. Proteomic Profile of Mouse Brain Aging Contributions to Mitochondrial Dysfunction, DNA Oxidative Damage, Loss of Neurotrophic Factor, and Synaptic and Ribosomal Proteins. Oxid Med Cell Longev 2020:5408452 (2020). PubMed: 32587661
- Stein J et al. Systematic expression analysis of the mitochondrial respiratory chain protein subunits identifies COX5B as a prognostic marker in clear cell renal cell carcinoma. Int J Urol 26:910-916 (2019). PubMed: 31280487
- Wang T et al. Pyridine nucleotide-disulphide oxidoreductase domain 2 (PYROXD2): Role in mitochondrial function. Mitochondrion 47:114-124 (2019). PubMed: 31170524
- Mot AI et al. Circumventing the Crabtree Effect: A method to induce lactate consumption and increase oxidative phosphorylation in cell culture. Int J Biochem Cell Biol 79:128-138 (2016). PubMed: 27590850