概述

  • 产品名称
    Anti-COX5B抗体[16H12H9]
    参阅全部 COX5B 一抗
  • 描述
    小鼠单克隆抗体[16H12H9] to COX5B
  • 经测试应用
    适用于: Flow Cyt, WBmore details
  • 种属反应性
    与反应: Mouse, Rat, Cow, Human
  • 免疫原

    Tissue/ cell preparation of Bovine COX5B from the heart.

  • 阳性对照
    • Isolated mitochondria from Human heart, Bovine heart, Rat heart, and Mouse heart
  • 常规说明

    This antibody clone is manufactured by Abcam.

    Product was previously marketed under the MitoSciences sub-brand.

性能

应用

Our Abpromise guarantee covers the use of ab110263 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Flow Cyt Use 1µg for 106 cells. ab170192-Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 14 kDa.

靶标

图片

  • All lanes : Anti-COX5B antibody [16H12H9] (ab110263) at 1 µg/ml

    Lane 1 : Human heart mitochondria at 5 µg
    Lane 2 : Bovine heart mitochondria at 1 µg
    Lane 3 : Rat heart mitochondria at 10 µg
    Lane 4 : Mouse heart mitochondria at 10 µg


    Predicted band size : 14 kDa
  • Overlay histogram showing HepG2 cells stained with ab110263 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab110263, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (ab91366, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HepG2 cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

文献

This product has been referenced in:
  • Kazak L  et al. UCP1 deficiency causes brown fat respiratory chain depletion and sensitizes mitochondria to calcium overload-induced dysfunction. Proc Natl Acad Sci U S A 114:7981-7986 (2017). WB ; Mouse . Read more (PubMed: 28630339) »
  • Srinivasan S  et al. Disruption of cytochrome c oxidase function induces the Warburg effect and metabolic reprogramming. Oncogene 35:1585-95 (2016). Read more (PubMed: 26148236) »

See all 10 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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