Anti-COX5B抗体[16H12H9] (ab110263)
Key features and details
- Mouse monoclonal [16H12H9] to COX5B
- Suitable for: Flow Cyt, WB
- Reacts with: Mouse, Rat, Cow, Human
- Isotype: IgG2b
概述
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产品名称
Anti-COX5B抗体[16H12H9]
参阅全部 COX5B 一抗 -
描述
小鼠单克隆抗体[16H12H9] to COX5B -
宿主
Mouse -
经测试应用
适用于: Flow Cyt, WBmore details -
种属反应性
与反应: Mouse, Rat, Cow, Human -
免疫原
Tissue, cells or virus. This information is considered to be commercially sensitive.
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阳性对照
- Isolated mitochondria from Human heart, Bovine heart, Rat heart, and Mouse heart
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常规说明
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Product was previously marketed under the MitoSciences sub-brand.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
存储溶液
pH: 7.5
Preservative: 0.02% Sodium azide
Constituent: HEPES buffered saline -
Concentration information loading...
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纯化说明
ab110263 was produced in vitro using hybridomas grown in serum-free medium, and then purified by biochemical fractionation. -
克隆
单克隆 -
克隆编号
16H12H9 -
同种型
IgG2b -
轻链类型
kappa -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab110263于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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Flow Cyt |
Use 1µg for 106 cells.
ab170192 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody. |
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WB |
Use a concentration of 1 µg/ml. Predicted molecular weight: 14 kDa.
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说明 |
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Flow Cyt
Use 1µg for 106 cells. ab170192 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody. |
WB
Use a concentration of 1 µg/ml. Predicted molecular weight: 14 kDa. |
靶标
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功能
This protein is one of the nuclear-coded polypeptide chains of cytochrome c oxidase, the terminal oxidase in mitochondrial electron transport. -
序列相似性
Belongs to the cytochrome c oxidase subunit 5B family. -
细胞定位
Mitochondrion inner membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 287012 Cow
- Entrez Gene: 1329 Human
- Entrez Gene: 12859 Mouse
- Entrez Gene: 94194 Rat
- Omim: 123866 Human
- SwissProt: P00428 Cow
- SwissProt: P10606 Human
- SwissProt: P19536 Mouse
see all -
别名
- COX 5B antibody
- COX5B antibody
- COX5B_HUMAN antibody
see all
图片
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All lanes : Anti-COX5B antibody [16H12H9] (ab110263) at 1 µg/ml
Lane 1 : Human heart mitochondria at 5 µg
Lane 2 : Bovine heart mitochondria at 1 µg
Lane 3 : Rat heart mitochondria at 10 µg
Lane 4 : Mouse heart mitochondria at 10 µg
Predicted band size: 14 kDa -
Overlay histogram showing HepG2 cells stained with ab110263 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab110263, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (ab91366, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HepG2 cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
数据表及文件
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SDS download
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Datasheet download
文献 (16)
ab110263 被引用在 16 文献中.
- Lee CQE et al. Coding and non-coding roles of MOCCI (C15ORF48) coordinate to regulate host inflammation and immunity. Nat Commun 12:2130 (2021). PubMed: 33837217
- Tang Y et al. Nicotinamide ameliorates energy deficiency and improves retinal function in Cav-1-/- mice. J Neurochem 157:550-560 (2021). PubMed: 33305362
- Signes A et al. APOPT1/COA8 assists COX assembly and is oppositely regulated by UPS and ROS. EMBO Mol Med 11:N/A (2019). PubMed: 30552096
- Ghosh J et al. Cigarette Smoke Toxins-Induced Mitochondrial Dysfunction and Pancreatitis Involves Aryl Hydrocarbon Receptor Mediated Cyp1 Gene Expression: Protective Effects of Resveratrol. Toxicol Sci 166:428-440 (2018). PubMed: 30165701
- Hasenan SM et al. Modulation of age related protein expression changes by gelam honey in cardiac mitochondrial rats. Exp Gerontol 113:1-9 (2018). PubMed: 30248357