Anti-COX4 + COX4L2抗体[10G8D12C12] (ab110261)

概述

  • 产品名称Anti-COX4 + COX4L2抗体[10G8D12C12]
    参阅全部 COX4 + COX4L2 一抗
  • 描述
    小鼠单克隆抗体[10G8D12C12] to COX4 + COX4L2
  • 经测试应用适用于: IHC-Fr, WB, Flow Cyt, ICC/IF, IHC-Pmore details
  • 种属反应性
    与反应: Cow, Human, Zebrafish
  • 免疫原

    Full length native Bovine protein, COX4 + COX4L2.

  • 阳性对照
    • Isolated mitochondria from Human heart, Bovine heart and HepG2 cells. Human skeletal muscle (frozen sections). Cultured Human embryonic lung derived fibroblasts (strain MRC5; fixed and permeabilized). Human colon tissue sections. HeLa cells.
  • 常规说明

    Product was previously marketed under the MitoSciences sub-brand.

性能

应用

Our Abpromise guarantee covers the use of ab110261 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-Fr 1/800.
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 20 kDa.
Flow Cyt Use a concentration of 1 µg/ml. ab170191-Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
ICC/IF Use a concentration of 5 µg/ml. (heat-induced antigen-retrieval improves signal)
IHC-P 1/200.

靶标

  • 相关性Cytochrome C Oxidase is located in the inner mitochondrial membrane and is the terminal enzyme complex of the mitochondrial electron transport chain. It collects electrons that are transferred from reduced cytochrome C and donates them to molecular oxygen, which is then reduced to water. It is composed of cytochrome A and cytochrome B, two copper atoms, and 13 different protein subunits, three of which are encoded by the mitochondrial DNA and ten others by nuclear DNA (mammals).
  • 细胞定位Mitochondrial inner membrane
  • 数据库链接
  • 别名
    • AL024441 antibody
    • COX 4 antibody
    • COX IV antibody
    • Cox4 antibody
    • Cox4a antibody
    • COX4B antibody
    • COX4I1 antibody
    • COX4I2 antibody
    • COX4L2 antibody
    • COXIV antibody
    • Cytochrome c oxidase polypeptide IV antibody
    • Cytochrome c oxidase subunit 4 isoform 1 mitochondrial antibody
    • Cytochrome C Oxidase subunit IV antibody
    • Cytochrome c oxidase subunit IV isoform 1 antibody
    • Cytochrome c oxidase subunit IV isoform 2 (lung) antibody
    • Cytochrome c oxydase subunit 4 antibody
    • dJ857M17.2 antibody
    • MGC105470 antibody
    • MGC72016 antibody
    see all

Anti-COX4 + COX4L2 antibody [10G8D12C12] 图像

  • All lanes : Anti-COX4 + COX4L2 antibody [10G8D12C12] (ab110261) at 1 µg/ml

    Lane 1 : Isolated mitochondria from Human heart at 5 µg
    Lane 2 : Isolated mitochondria from Bovine heart at 1 µg
    Lane 3 : Isolated mitochondria from HepG2 at 20 µg


    Predicted band size : 20 kDa
  • ab110261, at 1 µg/mL, staining COX4 + COX4L2 in HeLa cells by Immunohistochemistry (blue) and an isotype control antibody, at 1 µg/mL, staining of HeLa cells (red).
  • ab110261, at 10 µg/ml, staining COX4 + COX4L2 in cultured Human embryonic lung derived fibroblasts by Immunofluorescence. The cells (strain MRC5) were fixed, permeabilized and then labeled with ab110261 followed by an AlexaFluor® 488 conjugated Goat anti-mouse IgG2a isotype specific secondary antibody at 1 µg/ml.
  • ab110261, at 1/800, staining COX4 + COX4L2 in skeletal muscle tissue sections from a patient with a single large deletion of the mtDNA by Immunohistochemistry. The tissue was snap frozen and fixed in 4% PFA. Image kindly provided by Dr. J. Murphy and D. Turnbull, Mitochondrial Research Group, Newcastle University.
  • ab110261, at 1/200, showing COX4 + COX4L2 negative crypts in colon tissue sections from a normal ageing patient by Immunohistochemistry. The tissue was snap frozen and fixed in 4% PFA. Image kindly provided by Dr. J. Murphy and D. Turnbull, Mitochondrial Research Group, Newcastle University.

Anti-COX4 + COX4L2 antibody [10G8D12C12] (ab110261)参考文献

This product has been referenced in:
  • Yang T  et al. A small molecule TrkB/TrkC neurotrophin receptor co-activator with distinctive effects on neuronal survival and process outgrowth. Neuropharmacology 110:343-61 (2016). Read more (PubMed: 27334657) »
  • Qiu F  et al. Arginine starvation impairs mitochondrial respiratory function in ASS1-deficient breast cancer cells. Sci Signal 7:ra31 (2014). Human . Read more (PubMed: 24692592) »

See all 31 Publications for this product

Product Wall

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This antibody has been tested for immunohistochemistry by one of our collaborators. The optimal dilution varies depending on tissue types but 5ug/mL is recommended as a good starting point. Read More

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Application Western blot
Sample Human Cell lysate - whole cell
Loading amount 50 µg
Gel Running Conditions Reduced Denaturing (Reduced Denaturing (Reduced Denaturing (4-15%))
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
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Abcam user community

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提交于 Oct 09 2012

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Unfortunately, ab110261 has been tested and found to be unsuitable for use on mouse samples, and ab63947 was raised against a very short immunogen that shows only 69% homology with mouse. We would not expect either of ...

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Application Western blot
Sample Mouse Cell lysate - other (Isolated Mouse Liver Mitochondria)
Loading amount 20 µg
Specification Isolated Mouse Liver Mitochondria
Gel Running Conditions Reduced Denaturing (10% Bis Tris)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

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提交于 Dec 05 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"