Anti-COX2 / Cyclooxygenase 2抗体(ab88522)
Key features and details
- Mouse polyclonal to COX2 / Cyclooxygenase 2
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术,可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-COX2 / Cyclooxygenase 2抗体
参阅全部 COX2 / Cyclooxygenase 2 一抗 -
描述
小鼠多克隆抗体to COX2 / Cyclooxygenase 2 -
宿主
Mouse -
经测试应用
适用于: WB, ICC/IFmore details -
种属反应性
与反应: Human -
免疫原
Recombinant full length protein within Human COX2/ Cyclooxygenase 2. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
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阳性对照
- Jurkat cell lysate and COX2/Cyclooxygenase 2 transfected 293T cell lysate, HeLa cells
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
存储溶液
pH: 7.40
Constituent: 100% PBS -
Concentration information loading...
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纯度
Protein A purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab88522于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB | (1) |
Use a concentration of 1 µg/ml. Predicted molecular weight: 69 kDa.
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ICC/IF |
Use a concentration of 10 µg/ml.
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说明 |
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WB
Use a concentration of 1 µg/ml. Predicted molecular weight: 69 kDa. |
ICC/IF
Use a concentration of 10 µg/ml. |
靶标
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功能
Mediates the formation of prostaglandins from arachidonate. May have a role as a major mediator of inflammation and/or a role for prostanoid signaling in activity-dependent plasticity. -
通路
Lipid metabolism; prostaglandin biosynthesis. -
序列相似性
Belongs to the prostaglandin G/H synthase family.
Contains 1 EGF-like domain. -
翻译后修饰
S-nitrosylation by NOS2 (iNOS) activates enzme activity. S-nitrosylation may take place on different Cys residues in addition to Cys-561. -
细胞定位
Microsome membrane. Endoplasmic reticulum membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 5743 Human
- Omim: 600262 Human
- SwissProt: P35354 Human
- Unigene: 196384 Human
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别名
- COX 2 antibody
- COX-2 antibody
- COX2 antibody
see all
图片
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Anti-COX2 / Cyclooxygenase 2 antibody (ab88522) at 1 µg/ml + Jurkat cell lysate at 50 µg
Predicted band size: 69 kDa -
All lanes : Anti-COX2 / Cyclooxygenase 2 antibody (ab88522) at 1 µg/ml
Lane 1 : COX2 / Cyclooxygenase 2 transfected 293T cell lysate
Lane 2 : Non transfected 293T cell lysate
Lysates/proteins at 25 µg per lane.
Predicted band size: 69 kDa
Observed band size: 66 kDa why is the actual band size different from the predicted? -
ab88522 at 10 µg/ml staining COX2 / Cyclooxygenase 2 in paraformaldehyde fixed, permeabilised HeLa cells, by immunofluorescence.
实验方案
数据表及文件
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Datasheet download
文献 (1)
ab88522 被引用在 1 文献中.
- Zhang Z MiR-124-3p Suppresses Prostatic Carcinoma by Targeting PTGS2 Through the AKT/NF-?B Pathway. Mol Biotechnol 63:621-630 (2021). PubMed: 33893997