Anti-COX2 / Cyclooxygenase 2抗体(ab102005)


  • 产品名称Anti-COX2 / Cyclooxygenase 2抗体
    参阅全部 COX2 / Cyclooxygenase 2 一抗
  • 描述
    兔多克隆抗体to COX2 / Cyclooxygenase 2
  • 经测试应用适用于: IHC-P, WBmore details
  • 种属反应性
    与反应: Mouse, Human
    预测可用于: Rat, Sheep, Cow, Dog, Pig, Macaque Monkey
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 550 to the C-terminus of Human COX2/ Cyclooxygenase 2.

  • 阳性对照
    • This antibody gave a positive signal in Human Lung tissue lysate.



Our Abpromise guarantee covers the use of ab102005 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P Use a concentration of 5 µg/ml.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 69 kDa (predicted molecular weight: 69 kDa).


  • 功能Mediates the formation of prostaglandins from arachidonate. May have a role as a major mediator of inflammation and/or a role for prostanoid signaling in activity-dependent plasticity.
  • 通路Lipid metabolism; prostaglandin biosynthesis.
  • 序列相似性Belongs to the prostaglandin G/H synthase family.
    Contains 1 EGF-like domain.
  • 翻译后修饰S-nitrosylation by NOS2 (iNOS) activates enzme activity. S-nitrosylation may take place on different Cys residues in addition to Cys-561.
  • 细胞定位Microsome membrane. Endoplasmic reticulum membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • COX 2 antibody
    • COX-2 antibody
    • COX2 antibody
    • Cyclooxygenase 2 antibody
    • Cyclooxygenase 2b antibody
    • Cyclooxygenase antibody
    • Cyclooxygenase-2 antibody
    • Cyclooxygenase2 antibody
    • EC antibody
    • fj02a10 antibody
    • Glucocorticoid-regulated inflammatory cyclooxygenase antibody
    • Glucocorticoid-regulated inflammatory Prostaglandin G/H synthase antibody
    • GRIPGHS antibody
    • hCox 2 antibody
    • Macrophage activation-associated marker protein P71/73 antibody
    • OTTHUMP00000033524 antibody
    • PES-2 antibody
    • PGG/HS antibody
    • PGH synthase 2 antibody
    • PGH2_HUMAN antibody
    • PGHS 2 antibody
    • PGHS-2 antibody
    • PGHS2 antibody
    • PHS 2 antibody
    • PHS II antibody
    • PHS2 antibody
    • Prostaglandin endoperoxide synthase 2 (prostaglandin G/H synthase and cyclooxygenase) antibody
    • Prostaglandin endoperoxide synthase 2 antibody
    • Prostaglandin G/H synthase 2 antibody
    • Prostaglandin G/H synthase 2 precursor antibody
    • Prostaglandin G/H synthase and cyclooxygenase antibody
    • Prostaglandin G/H synthase antibody
    • Prostaglandin H2 synthase 2 antibody
    • prostaglandin-endoperoxide synthase 2 (prostaglandin G/H synthase and cyclooxygenase) antibody
    • Prostaglandin-endoperoxide synthase 2 antibody
    • PTGS2 antibody
    • ptgs2a antibody
    • TIS10 antibody
    • TIS10 protein antibody
    • unp1239 antibody
    • wu:fj02a10 antibody
    see all

Anti-COX2 / Cyclooxygenase 2 antibody 图像

  • Anti-COX2 / Cyclooxygenase 2 antibody (ab102005) at 1 µg/ml + Lung (Human) Tissue Lysate at 10 µg

    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 69 kDa
    Observed band size : 69 kDa
    Additional bands at : 43 kDa,52 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 6 minutes
  • IHC image of COX2 / Cyclooxygenase 2 staining in Human breast cancer formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab102005, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Anti-COX2 / Cyclooxygenase 2 antibody (ab102005)参考文献

This product has been referenced in:
  • Cui TZ  et al. Modulation of the respiratory supercomplexes in yeast: enhanced formation of cytochrome oxidase increases the stability and abundance of respiratory supercomplexes. J Biol Chem 289:6133-41 (2014). WB ; Saccharomyces cerevisiae . Read more (PubMed: 24421313) »
  • Atkinson A  et al. The LYR protein Mzm1 functions in the insertion of the Rieske Fe/S protein in yeast mitochondria. Mol Cell Biol 31:3988-96 (2011). WB ; Saccharomyces cerevisiae . Read more (PubMed: 21807901) »

See all 2 Publications for this product

Product Wall

Application Western blot
Sample Dog Cell lysate - whole cell (canine dermal fibroblasts)
Gel Running Conditions Reduced Denaturing (7.5% gel)
Loading amount 10 µg
Treatment canine recombinant IL-1β
Specification canine dermal fibroblasts
Blocking step Block Ace as blocking agent for 50 minute(s) · Concentration: 100%

rei nakano

Verified customer

提交于 Nov 12 2015

Application Western blot
Loading amount 20 µg
Gel Running Conditions Reduced Denaturing (10%)
Sample Dog Cell lysate - whole cell (Canine Chondrocytes)
Specification Canine Chondrocytes
Treatment 10ng/ml IL1B, and 100uM TNFa antagonist, for 4 hours.
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C

Abcam user community

Verified customer

提交于 Oct 27 2014

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (pancreas)
Specification pancreas
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Sodium Citrate
Permeabilization No
Blocking step GOAT BLOCK = 10mM Tris pH 7.4, 100mM MgCl2, 0.5% Tween, 1% BSA, 5% normal goat serum (IN WATER) as blocking agent for 20 minute(s) · Concentration: 100% · Temperature: 25°C

Abcam user community

Verified customer

提交于 Apr 11 2013

Thank you for your reply.

Indeed, the other antibodies mentioned are only tested for WB so far, however, they are the antibodies against these targets that have the highest probability to cross-react with hamster due to sequence similarity o...

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Thank you very much for contacting us.

I am happy to let you know that we have the following antibodies available:

COX-2: ab102005: Click here (or use the following:
COX-1: a...

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