重组Anti-COX2 / Cyclooxygenase 2抗体[SP21] (ab16701)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [SP21] to COX2 / Cyclooxygenase 2
- Suitable for: IHC-P
- Reacts with: Human
概述
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产品名称
Anti-COX2 / Cyclooxygenase 2抗体[SP21]
参阅全部 COX2 / Cyclooxygenase 2 一抗 -
描述
兔单克隆抗体[SP21] to COX2 / Cyclooxygenase 2 -
宿主
Rabbit -
经测试应用
适用于: IHC-Pmore details
不适用于: WB -
种属反应性
与反应: Human
预测可用于: Mouse, Rat -
免疫原
Synthetic peptide within Rat COX2/ Cyclooxygenase 2 (C terminal). The exact sequence is proprietary.
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阳性对照
- IHC-P: Human colon carcinoma tissue.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
This product is FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7.20
Preservative: 0.1% Sodium azide
Constituents: 1% BSA, PBS -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
SP21 -
同种型
IgG -
研究领域
相关产品
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Isotype control
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Positive Controls
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab16701于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
IHC-P | (1) |
1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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说明 |
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IHC-P
1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
靶标
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功能
Mediates the formation of prostaglandins from arachidonate. May have a role as a major mediator of inflammation and/or a role for prostanoid signaling in activity-dependent plasticity. -
通路
Lipid metabolism; prostaglandin biosynthesis. -
序列相似性
Belongs to the prostaglandin G/H synthase family.
Contains 1 EGF-like domain. -
翻译后修饰
S-nitrosylation by NOS2 (iNOS) activates enzme activity. S-nitrosylation may take place on different Cys residues in addition to Cys-561. -
细胞定位
Microsome membrane. Endoplasmic reticulum membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 5743 Human
- Entrez Gene: 19225 Mouse
- Entrez Gene: 29527 Rat
- Omim: 600262 Human
- SwissProt: P35354 Human
- SwissProt: Q05769 Mouse
- SwissProt: P35355 Rat
- Unigene: 196384 Human
see all -
别名
- COX 2 antibody
- COX-2 antibody
- COX2 antibody
see all
图片
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Immunohistochemical analysis of paraffin-embedded Human colon carcinoma labeling COX2/Cyclooxygenase 2 with ab16701 at 1/100 (6 µg/ml). The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab16701 for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. DAB was used as the chromogen. Counterstained with Hematoxylin and mounted with DPX.
数据表及文件
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SDS download
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Datasheet download
文献 (7)
ab16701 被引用在 7 文献中.
- Madhavan MK et al. Aberrant uterine folding in mice disrupts implantation chamber formation and alignment of embryo-uterine axes. Development 149:N/A (2022). PubMed: 35575097
- Szlendak M et al. Expression of cyclooxygenase-2 and mucin 1 in colorectal cancer. Mol Clin Oncol 13:52 (2020). PubMed: 32874582
- Keren L et al. MIBI-TOF: A multiplexed imaging platform relates cellular phenotypes and tissue structure. Sci Adv 5:eaax5851 (2019). Mass Cytometry . PubMed: 31633026
- Peng YJ et al. Adiponectin and adiponectin receptor 1 overexpression enhance inflammatory bowel disease. J Biomed Sci 25:24 (2018). PubMed: 29540173
- Szylberg L et al. Expression of COX-2, IL-1ß, TNF-a and IL-4 in epithelium of serrated adenoma, adenoma and hyperplastic polyp. Arch Med Sci 12:172-8 (2016). PubMed: 26925134
- Fujita M et al. COX-2 blockade suppresses gliomagenesis by inhibiting myeloid-derived suppressor cells. Cancer Res 71:2664-74 (2011). PubMed: 21324923
- Lu TC et al. Analgesic and Anti-Inflammatory Activities of the Methanol Extract from Pogostemon cablin. Evid Based Complement Alternat Med : (2009). PubMed: 19933324