Anti-COX2 / Cyclooxygenase 2抗体(ab52237)

概述

  • 产品名称
    Anti-COX2 / Cyclooxygenase 2抗体
    参阅全部 COX2 / Cyclooxygenase 2 一抗
  • 描述
    兔多克隆抗体to COX2 / Cyclooxygenase 2
  • 经测试应用
    适用于: ELISA, WB, IHC-P, ICC/IFmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide corresponding to Human COX2/ Cyclooxygenase 2.

  • 阳性对照
    • Extracts from A549 cells. This antibody gave a positive result in IHC in the following FFPE tissue: Human lung adenocarcinoma.

应用

Our Abpromise guarantee covers the use of ab52237 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA 1/20000.
WB 1/500 - 1/1000. Detects a band of approximately 69 kDa (predicted molecular weight: 69 kDa).
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF 1/100 - 1/500.

靶标

  • 功能
    Mediates the formation of prostaglandins from arachidonate. May have a role as a major mediator of inflammation and/or a role for prostanoid signaling in activity-dependent plasticity.
  • 通路
    Lipid metabolism; prostaglandin biosynthesis.
  • 序列相似性
    Belongs to the prostaglandin G/H synthase family.
    Contains 1 EGF-like domain.
  • 翻译后修饰
    S-nitrosylation by NOS2 (iNOS) activates enzme activity. S-nitrosylation may take place on different Cys residues in addition to Cys-561.
  • 细胞定位
    Microsome membrane. Endoplasmic reticulum membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • COX 2 antibody
    • COX-2 antibody
    • COX2 antibody
    • Cyclooxygenase 2 antibody
    • Cyclooxygenase 2b antibody
    • Cyclooxygenase antibody
    • Cyclooxygenase-2 antibody
    • Cyclooxygenase2 antibody
    • EC 1.14.99.1 antibody
    • fj02a10 antibody
    • Glucocorticoid-regulated inflammatory cyclooxygenase antibody
    • Glucocorticoid-regulated inflammatory Prostaglandin G/H synthase antibody
    • GRIPGHS antibody
    • hCox 2 antibody
    • Macrophage activation-associated marker protein P71/73 antibody
    • OTTHUMP00000033524 antibody
    • PES-2 antibody
    • PGG/HS antibody
    • PGH synthase 2 antibody
    • PGH2_HUMAN antibody
    • PGHS 2 antibody
    • PGHS-2 antibody
    • PGHS2 antibody
    • PHS 2 antibody
    • PHS II antibody
    • PHS2 antibody
    • Prostaglandin endoperoxide synthase 2 (prostaglandin G/H synthase and cyclooxygenase) antibody
    • Prostaglandin endoperoxide synthase 2 antibody
    • Prostaglandin G/H synthase 2 antibody
    • Prostaglandin G/H synthase 2 precursor antibody
    • Prostaglandin G/H synthase and cyclooxygenase antibody
    • Prostaglandin G/H synthase antibody
    • Prostaglandin H2 synthase 2 antibody
    • prostaglandin-endoperoxide synthase 2 (prostaglandin G/H synthase and cyclooxygenase) antibody
    • Prostaglandin-endoperoxide synthase 2 antibody
    • PTGS2 antibody
    • ptgs2a antibody
    • TIS10 antibody
    • TIS10 protein antibody
    • unp1239 antibody
    • wu:fj02a10 antibody
    see all

图片

  • Anti-COX2 / Cyclooxygenase 2 antibody (ab52237) at 1/1000 dilution + Human primary myometrial cells whole cell lysate at 20 µg

    Secondary
    Anti-rabbit IgG, HRP-linked at 1/4000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 69 kDa
    Observed band size : 70 kDa (why is the actual band size different from the predicted?)


    Exposure time : 1 minute

    See Abreview

  • IHC image of COX2 / Cyclooxygenase 2 staining in Human lung adenocarcinoma formalin fixed paraffin embedded tissue section, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab52237, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • All lanes : Anti-COX2 / Cyclooxygenase 2 antibody (ab52237) at 1/500 dilution

    Lane 1 : A549 (Human lung adenocarcinoma epithelial)
    Lane 2 : A549 (Human lung adenocarcinoma epithelial) with Synthesized Peptide


    Predicted band size : 69 kDa
  • Immunocytochemistry/immunofluorescence analysis of A549 (Human lung adenocarcinoma epithelial) cells labelling COX2 / Cyclooxygenase 2 with ab52237 at dilution of 1/100. No staining was abserved when blocked with synthesized peptide as shown in the inset.

  • Anti-COX2 / Cyclooxygenase 2 antibody (ab52237) (Human mesothelioma whole cell lysate.)

    Secondary
    Goat anti-rabbit HRP

    Predicted band size : 69 kDa

    This image is courtesy of an anonymous Abreview

    Please see accompanying abreview for additional information

    See Abreview

文献

This product has been referenced in:
  • Kuanpradit C  et al. Attenuation of UV-B exposure-induced inflammation by abalone hypobranchial gland and gill extracts. Int J Mol Med 39:1083-1090 (2017). WB ; Human . Read more (PubMed: 28358420) »
  • Mehdawi LM  et al. A potential anti-tumor effect of leukotriene C4 through the induction of 15-hydroxyprostaglandin dehydrogenase expression in colon cancer cells. Oncotarget 8:35033-35047 (2017). Read more (PubMed: 28402256) »

See all 20 Publications for this product

客户评价及客户问答

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Tissue lysate - whole (Pooled endometrial tissue)
Gel Running Conditions
Reduced Denaturing (12%)
Loading amount
20 µg
Specification
Pooled endometrial tissue
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Abcam user community

Verified customer

提交于 Jun 30 2017

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (primary myometrial cells)
Loading amount
20 µg
Specification
primary myometrial cells
Treatment
not serum starved
Gel Running Conditions
Reduced Denaturing (8% Bis-Tris)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Username

Abcam user community

Verified customer

提交于 Oct 09 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Rat Tissue lysate - whole (Lung)
Loading amount
30 µg
Specification
Lung
Gel Running Conditions
Reduced Denaturing (12%)
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 20°C
Username

Mr. Weibin Fang

Verified customer

提交于 Feb 10 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (epithelial)
Loading amount
25 µg
Specification
epithelial
Gel Running Conditions
Non-reduced Denaturing (10%)
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 Aug 05 2009

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (Mesothelioma)
Loading amount
20 µg
Specification
Mesothelioma
Gel Running Conditions
Non-reduced Denaturing (12% Tris-HEPES-SDS)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Username

Abcam user community

Verified customer

提交于 Mar 06 2009

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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