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Synthetic peptide corresponding to Human COX2/ Cyclooxygenase 2.
Our Abpromise guarantee covers the use of ab52237 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/1000. Detects a band of approximately 69 kDa (predicted molecular weight: 69 kDa).|
|IHC-P||Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|ICC/IF||1/100 - 1/500.|
IHC image of COX2 / Cyclooxygenase 2 staining in Human lung adenocarcinoma formalin fixed paraffin embedded tissue section, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab52237, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
Immunocytochemistry/immunofluorescence analysis of A549 (Human lung adenocarcinoma epithelial) cells labelling COX2 / Cyclooxygenase 2 with ab52237 at dilution of 1/100. No staining was abserved when blocked with synthesized peptide as shown in the inset.
This image is courtesy of an anonymous AbreviewPlease see accompanying abreview for additional information
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