概述

  • 产品名称Anti-COX17抗体
    参阅全部 COX17 一抗
  • 描述
    兔多克隆抗体to COX17
  • 经测试应用适用于: ELISA, WB, IHC-Pmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat
  • 免疫原

    Synthetic peptide derived from the N-terminal of human COX17.

  • 阳性对照
    • Human liver carcinoma tissue. Extracts from HeLa, HepG2, 293 and COLO cells.

性能

应用

Our Abpromise guarantee covers the use of ab69611 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA 1/5000.
WB 1/500 - 1/1000. Detects a band of approximately <19 kDa (predicted molecular weight: 7 kDa).
IHC-P 1/50 - 1/100.

靶标

  • 功能Copper chaperone for cytochrome c oxidase (COX). Binds two copper ions and deliver them to the Cu(A) site of COX.
  • 组织特异性Ubiquitous.
  • 序列相似性Belongs to the COX17 family.
  • 细胞定位Mitochondrion intermembrane space.
  • Information by UniProt
  • 数据库链接
  • 别名
    • COX 17 antibody
    • COX17 antibody
    • COX17 cytochrome c oxidase assembly homolog (S. cerevisiae) antibody
    • COX17 cytochrome c oxidase assembly homolog antibody
    • COX17 homolog cytochrome c oxidase assembly protein antibody
    • COX17_HUMAN antibody
    • cytochrome c oxidase assembly protein cox17 homolog antibody
    • Cytochrome c oxidase copper chaperone antibody
    • Human homolog of yeast mitochondrial copper recruitment antibody
    • MGC104397 antibody
    • MGC117386 antibody
    • OTTHUMP00000215284 antibody
    • OTTHUMP00000215285 antibody
    see all

Anti-COX17 antibody 图像

  • All lanes : Anti-COX17 antibody (ab69611) at 1/500 dilution

    Lane 1 : Extracts from HeLa cells
    Lane 2 : Extracts from HepG2 cells
    Lane 3 : Extracts from COLO cells
    Lane 4 : Extracts from 293 cells
    Lane 5 : Extracts from COLO cells with immunising peptide at 5 µg

    Lysates/proteins at 5 µg per lane.


    Predicted band size : 7 kDa
    Observed band size : <19 kDa (why is the actual band size different from the predicted?)
  • ab69611, at 1/50 dilution, staining human liver carcinoma tissue in the presence or absence of the immunising peptide.
  • ICC/IF image of ab69611 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab69611, 1µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-COX17 antibody (ab69611)参考文献

ab69611 has not yet been referenced specifically in any publications.

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