概述

  • 产品名称Anti-COX10抗体
    参阅全部 COX10 一抗
  • 描述
    兔多克隆抗体to COX10
  • 经测试应用适用于: ICC/IF, WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Pig, Drosophila melanogaster, Zebrafish
  • 免疫原

    Synthetic peptide within residues: APGPFDWPCF LLTSVGTGLA SCAANSINQF FEVPFDSNMN RTKNRPLVRG , corresponding to internal sequence amino acids 180-229 of Human COX10 (NP_001294)

  • 阳性对照
    • Human fetal lung lysate. This antibody gave a positive result when used in the following formaldehyde fixed cell lines: HepG2.

性能

应用

Our Abpromise guarantee covers the use of ab84053 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use a concentration of 5 µg/ml.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 49 kDa (predicted molecular weight: 49 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

靶标

  • 功能Converts protoheme IX and farnesyl diphosphate to heme O.
  • 疾病相关Defects in COX10 are a cause of mitochondrial complex IV deficiency (MT-C4D) [MIM:220110]; also known as cytochrome c oxidase deficiency. A disorder of the mitochondrial respiratory chain with heterogeneous clinical manifestations, ranging from isolated myopathy to severe multisystem disease affecting several tissues and organs. Features include hypertrophic cardiomyopathy, hepatomegaly and liver dysfunction, hypotonia, muscle weakness, excercise intolerance, developmental delay, delayed motor development and mental retardation. A subset of patients manifest Leigh syndrome.
    Defects in COX10 are a cause of Leigh syndrome (LS) [MIM:256000]. LS is a severe neurological disorder characterized by bilaterally symmetrical necrotic lesions in subcortical brain regions.
  • 序列相似性Belongs to the ubiA prenyltransferase family.
  • 细胞定位Mitochondrion membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • 2410004F01Rik antibody
    • AU042636 antibody
    • COX10 antibody
    • COX10_HUMAN antibody
    • Cytochrome c oxidase assembly protein antibody
    • Cytochrome c oxidase subunit X antibody
    • Heme A farnesyltransferase antibody
    • Heme O synthase antibody
    • OTTMUSP00000006085 antibody
    • Protoheme IX farnesyltransferase, mitochondrial antibody
    • Protoheme IX farnesyltransferase, mitochondrial precursor antibody
    • RP23-78H18.1 antibody
    see all

Anti-COX10 antibody 图像

  • ICC/IF image of ab84053 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab84053 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Anti-COX10 antibody (ab84053) at 1 µg/ml (in 5% skim milk / PBS buffer) + Human fetal lung lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 49 kDa
    Observed band size : 49 kDa
  • All lanes : Anti-COX10 antibody (ab84053) at 1/1000 dilution

    Lane 1 : Isolated mitochondria from wild type mice at 15 µg
    Lane 2 : Isolated mitochondria from wild type mice at 30 µg


    Predicted band size : 49 kDa

Anti-COX10 antibody (ab84053)参考文献

ab84053 has not yet been referenced specifically in any publications.

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