概述

  • 产品名称Anti-COX IV抗体
    参阅全部 COX IV 一抗
  • 描述
    兔多克隆抗体to COX IV
  • 经测试应用适用于: WB, IHC-P, ICC/IFmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Pig, Chimpanzee
  • 免疫原

    A region within synthetic peptide: AISTSVCVRA HESVVKSEDF SLPAYMDRRD HPLPEVAHVK HLSASQKALK, corresponding to amino acids 14-63 of Human COX IV

  • 阳性对照
    • HepG2 cell lysate. Human heart tissue. Human kidney tissue. This antibody gave a positive result when used in the following methanol fixed cell lines: mcf-7.

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应用

Our Abpromise guarantee covers the use of ab66739 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1.25 µg/ml. Detects a band of approximately 18 kDa (predicted molecular weight: 20 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P Use a concentration of 2 µg/ml.
ICC/IF Use a concentration of 5 µg/ml.

靶标

  • 功能This protein is one of the nuclear-coded polypeptide chains of cytochrome c oxidase, the terminal oxidase in mitochondrial electron transport.
  • 组织特异性Ubiquitous.
  • 序列相似性Belongs to the cytochrome c oxidase IV family.
  • 细胞定位Mitochondrion inner membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • AL024441 antibody
    • COX 4 antibody
    • COX IV 1 antibody
    • COX IV antibody
    • COX IV-1 antibody
    • Cox4 antibody
    • COX41_HUMAN antibody
    • Cox4a antibody
    • COX4B antibody
    • COX4I1 antibody
    • COX4I2 antibody
    • COX4L2 antibody
    • COXIV antibody
    • Cytochrome c oxidase polypeptide IV antibody
    • Cytochrome c oxidase subunit 4 isoform 1 mitochondrial antibody
    • Cytochrome c oxidase subunit 4 isoform 1, mitochondrial antibody
    • Cytochrome C Oxidase subunit IV antibody
    • Cytochrome c oxidase subunit IV isoform 1 antibody
    • Cytochrome c oxidase subunit IV isoform 2 (lung) antibody
    • Cytochrome c oxydase subunit 4 antibody
    • dJ857M17.2 antibody
    • MGC105470 antibody
    • MGC72016 antibody
    see all

Anti-COX IV antibody 图像



  • Predicted band size : 20 kDa
    Western blot analysis of HeLa (lane 1) 293T (lane 2) K562 (lane 3) MDA-MB-231 (lane 4) cell lysates (50µg/lane) labeling COX IV with ab66739 at 1:1000. A HRP-conjugated anti-rabbit (1:1000) was used as the secondary antibody.
  • ab66739, at 2 µg/ml, staining COX IV in human kidney tissue by immunohistochemistry on paraffin-embedded tissue. Positively stained cells are epithelial cells of the renal tubule.

  • ab66739, at 2 µg/ml, staining COX IV in Human heart tissue by immunohistochemistry on paraffin-embedded tissue. The arrows indicate positively stained myocardial cells.

  • ICC/IF image of ab66739 stained MCF-7 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab66739 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Anti-COX IV antibody (ab66739) at 1.25 µg/ml + HepG2 cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 20 kDa
    Observed band size : <21 kDa (why is the actual band size different from the predicted?)
    Antibody diltued in 5% skim milk / PBS buffer. Gel concentration 12%.
  • All lanes : Anti-COX IV antibody (ab66739)

    Lane 1 : Lysate prepared from human liver
    Lane 2 : Lysate prepared from rat liver
    Lane 3 : Lysate prepared from wild type mouse liver
    Lane 4 : Lysate prepared from AMPKa1+2-/- mouse liver
    Lane 5 : Lysate prepared from human muscle
    Lane 6 : Lysate prepared from rat muscle
    Lane 7 : Lysate prepared from mouse muscle


    Predicted band size : 20 kDa
    A small piece (~30mg) of every organ was homogenized in a 6:1 (v/w) ratio of ice-cold buffer containing: 50 mM 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (pH 7.6), 50 mM sodium fluoride, 50 mM potassium chloride, 5 mM sodium pyrophosphate, 1 mM ethylenediaminetetraacetic acid, 1 mM ethylene glycol tetraacetic acid, 5 mM ß-glycerophosphate, 1 mM sodium vanadate, 1 mM dithiothreitol, 1% nonyl phenoxypolyethoxylethanol (Tergitol-type NP40) and protease inhibitors cocktail. Homogenates were centrifuged (13,200 rpm; 15 min, 4°C) and the protein content of the supernatant was determined using a bicinchoninic acid (BCA) protein assay kit.
    Proteins (30 µg) were separated by either 12.5 or 10% SDS-PAGE followed by transfer to a polyvinylidene fluoride transfer membrane. Membranes were blocked for 1 h at room temperature in tris-buffered saline tween-20 buffer with 5% non-fat dry milk followed by an overnight incubation with antibodies. Blots were then incubated with horseradish

Anti-COX IV antibody (ab66739)参考文献

This product has been referenced in:
  • Snyder AM  et al. Mitochondrial ferritin in the substantia nigra in restless legs syndrome. J Neuropathol Exp Neurol 68:1193-9 (2009). Read more (PubMed: 19816198) »

See 1 Publication for this product

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