Anti-COX IV抗体(ab64885)


  • 产品名称Anti-COX IV抗体
    参阅全部 COX IV 一抗
  • 描述
    兔多克隆抗体to COX IV
  • 特异性ab64885 detects endogenous levels of total COX41 protein.
  • 经测试应用适用于: ICC/IF, IHC-P, ELISA, WBmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthetic peptide corresponding to Human COX IV (N terminal).

  • 阳性对照
    • Human colon carcinoma tissue, extracts from A549 cells.


Our Abpromise guarantee covers the use of ab64885 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use a concentration of 1 - 5 µg/ml.
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
ELISA 1/20000.
WB 1/500 - 1/1000. Detects a band of approximately 20 kDa (predicted molecular weight: 20 kDa).


  • 功能This protein is one of the nuclear-coded polypeptide chains of cytochrome c oxidase, the terminal oxidase in mitochondrial electron transport.
  • 组织特异性Ubiquitous.
  • 序列相似性Belongs to the cytochrome c oxidase IV family.
  • 细胞定位Mitochondrion inner membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • AL024441 antibody
    • COX 4 antibody
    • COX IV 1 antibody
    • COX IV antibody
    • COX IV-1 antibody
    • Cox4 antibody
    • COX41_HUMAN antibody
    • Cox4a antibody
    • COX4B antibody
    • COX4I1 antibody
    • COX4I2 antibody
    • COX4L2 antibody
    • COXIV antibody
    • Cytochrome c oxidase polypeptide IV antibody
    • Cytochrome c oxidase subunit 4 isoform 1 mitochondrial antibody
    • Cytochrome c oxidase subunit 4 isoform 1, mitochondrial antibody
    • Cytochrome C Oxidase subunit IV antibody
    • Cytochrome c oxidase subunit IV isoform 1 antibody
    • Cytochrome c oxidase subunit IV isoform 2 (lung) antibody
    • Cytochrome c oxydase subunit 4 antibody
    • dJ857M17.2 antibody
    • MGC105470 antibody
    • MGC72016 antibody
    see all

Anti-COX IV antibody 图像

  • This image shows human colon carcinoma tissue stained with ab64885 at 1/50-1/100 dilution; with (+) and without (-) the immunising peptide.
  • All lanes : Anti-COX IV antibody (ab64885) at 1/500 dilution

    Lane 1 : extracts from A549 cells (5-30µg total protein)
    Lane 2 : extracts from A549 cells (5-30µg total protein) and 5-10µg of the immunising peptide.

    Predicted band size : 20 kDa
    Observed band size : 20 kDa
  • ICC/IF image of ab64885 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab64885, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-COX IV antibody (ab64885)参考文献

ab64885 has not yet been referenced specifically in any publications.

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Immunohistochemistry on frozen sections is the best technique for snap frozen sections. Please click the link below to know about IHC-FR

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