Anti-COX IV抗体[20E8C12] (ab14744)

概述

  • 产品名称Anti-COX IV抗体[20E8C12]
    参阅全部 COX IV 一抗
  • 描述
    小鼠单克隆抗体[20E8C12] to COX IV
  • 经测试应用适用于: Flow Cyt, IHC-FoFr, WB, ICC/IF, IHC-Fr, IHC-P, IPmore details
  • 种属反应性
    与反应: Mouse, Rat, Hamster, Cow, Human, Drosophila melanogaster, Zebrafish
  • 免疫原

    Tissue, cells or virus corresponding to Cow COX IV.

  • 阳性对照
    • Human, bovine, murine and rat heart mitochondria.
  • 常规说明

    This antibody makes an effective loading control for mitochondria. COXIV is generally expressed at a consistent high level. However, be aware that many proteins run at the same 16kD size as COXIV - our VDAC1 / Porin antibody makes a good alternative mitochondrial loading control for proteins of this size. Some caution is required when using this antibody as a loading control as COXIV expression can vary under some manipulations. An alternative mitochondrial loading control is Rabbit polyclonal to COX IV antibody (ab16056).

     

    Alternative versions available:

    Anti-COX IV antibody (Alexa Fluor® 488) [20E8C12] (ab197906)

    Anti-COX IV antibody (Alexa Fluor® 647) [20E8C12] (ab197651)

    Anti-COX IV antibody (HRP) [20E8C12] (ab197920)

性能

应用

Our Abpromise guarantee covers the use of ab14744 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Flow Cyt Use 0.5µg for 106 cells. ab170191-Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
IHC-FoFr Use at an assay dependent concentration. PubMed: 18698340
WB Use a concentration of 1 µg/ml. Detects a band of approximately 17 kDa.
ICC/IF Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration.
IP Use at an assay dependent concentration.

靶标

  • 功能This protein is one of the nuclear-coded polypeptide chains of cytochrome c oxidase, the terminal oxidase in mitochondrial electron transport.
  • 组织特异性Ubiquitous.
  • 序列相似性Belongs to the cytochrome c oxidase IV family.
  • 细胞定位Mitochondrion inner membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • AL024441 antibody
    • COX 4 antibody
    • COX IV 1 antibody
    • COX IV antibody
    • COX IV-1 antibody
    • Cox4 antibody
    • COX41_HUMAN antibody
    • Cox4a antibody
    • COX4B antibody
    • COX4I1 antibody
    • COX4I2 antibody
    • COX4L2 antibody
    • COXIV antibody
    • Cytochrome c oxidase polypeptide IV antibody
    • Cytochrome c oxidase subunit 4 isoform 1 mitochondrial antibody
    • Cytochrome c oxidase subunit 4 isoform 1, mitochondrial antibody
    • Cytochrome C Oxidase subunit IV antibody
    • Cytochrome c oxidase subunit IV isoform 1 antibody
    • Cytochrome c oxidase subunit IV isoform 2 (lung) antibody
    • Cytochrome c oxydase subunit 4 antibody
    • dJ857M17.2 antibody
    • MGC105470 antibody
    • MGC72016 antibody
    see all

Anti-COX IV antibody [20E8C12] 图像

  • ab14744 at 1/100 staining MCF-10A cells (human mammary epithelial cell line) by ICC/IF. The cells were methanol/acetone fixed at -20C for 5 minutes, blocked with BSA and then incubated with the antibody for 16 hours. The positive tissue was colocalised with a mitotracker (mitotrackers are a series of patented mt-selective stains that are concentrated by active mt and well retained during cell fixation). An Alexa-Fluor ® 488 conjugated goat anti-mouse antibody was used as the secondary. The image shows COXIV staining in green and DAPI staining in blue.

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  • Anti-COX IV antibody [20E8C12] (ab14744) at 1/1000 dilution + Fruit fly (Drosophila melanogaster) heart tube tissue lysate at 10 µg

    Secondary
    HRP-conjugate Goat anti-mouse IgG polyclonal at 1/4000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Exposure time : 1 minute

    This image is courtesy of an anonymous Abreview

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  • Overlay histogram showing HeLa cells stained with ab14744 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab14744, 0.5µg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1](ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

  • All lanes : Anti-COX IV antibody [20E8C12] (ab14744) at 1/5000 dilution

    Lane 1 : Cytoplasmic fraction mouse NIH/3T3 cell lysate
    Lane 2 : Nuclear fraction mouse NIH/3T3 cell lysate

    Secondary
    HRP conjugated goat anti-mouse antibody
    Developed using the ECL technique

    Performed under reducing conditions.

    Observed band size : 16 kDa (why is the actual band size different from the predicted?)


    Exposure time : 3 minutes
    This image is courtesy of an Abreview submitted by Camilla Skjerpen on 4 July 2005.

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  • ab14744 staining COX IV in rat brain tissue sections by Immunohistochemistry (PFA perfusion fixed frozen sections). Rats were anesthetized and intracardially perfused with 500 ml of normal saline at room temperature, followed by 500 ml of ice-cold, freshly made 4% paraformaldehyde in phosphate buffer (PB, 0.1 M, pH 7.4). A Cy3 conjugated anti mouse antibody was used as secondary. 

  • All lanes : Anti-COX IV antibody [20E8C12] (ab14744) at 1 µg/ml

    Lane 1 : Isolated mitochondria from human heart at 5 µg
    Lane 2 : Isolated mitochondria from bovine heart at 1 µg
    Lane 3 : Isolated mitochondria from rat heart at 10 µg
    Lane 4 : Isolated mitochondria from murine heart at 10 µg


    Observed band size : 16 kDa (why is the actual band size different from the predicted?)
  • ab14744 staining COX IV - Mitochondrial Loading in the HEK293 cell line from Human embryonic kidney by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with Triton X-100 1% in PBS and blocked with 5% serum for 60 minutes at 21°C. Samples were incubated with primary antibody (1/200 in 5% Goat serum) for 2 hours. An Alexa Fluor®546-conjugated Goat anti-mouse IgG polyclonal was used as the secondary antibody.

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Anti-COX IV antibody [20E8C12] (ab14744)参考文献

This product has been referenced in:
  • Sakakibara I  et al. Six1 homeoprotein drives myofiber type IIA specialization in soleus muscle. Skelet Muscle 6:30 (2016). WB ; Mouse . Read more (PubMed: 27597886) »
  • Hiniker A  et al. Axial mitochondrial myopathy in a patient with rapidly progressive adult-onset scoliosis. Acta Neuropathol Commun 2:137 (2014). IHC-Fr ; Human . Read more (PubMed: 25223649) »

See all 91 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (HEK293)
Gel Running Conditions Reduced Denaturing
Loading amount 50 µg
Specification HEK293
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: rt°C
Username

Dr. alex lee

Verified customer

提交于 Nov 24 2016

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Cell lysate - whole cell (MEF)
Gel Running Conditions Reduced Denaturing
Loading amount 50 µg
Specification MEF
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 Nov 24 2016

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Mouse Cell (MEF)
Permeabilization Yes - 0.1% triton x-100
Specification MEF
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
Fixative Paraformaldehyde
Username

Abcam user community

Verified customer

提交于 Nov 24 2016

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (HEK293)
Permeabilization Yes - 0.1% triton x-100
Specification HEK293
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
Fixative Paraformaldehyde
Username

Abcam user community

Verified customer

提交于 Nov 24 2016

Application Western blot
Sample Sheep Cell lysate - other (preadipocyte mitochondrial isolates v. cytoplasmic)
Gel Running Conditions Reduced Denaturing (10% PAG)
Loading amount 10 µg
Specification preadipocyte mitochondrial isolates v. cytoplasmic
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Username

Mr. Jeremy Gingrich

Verified customer

提交于 Oct 13 2016

Application Immunocytochemistry/ Immunofluorescence
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: RT°C
Sample Chinese Hamster Cell (CHO cell)
Specification CHO cell
Permeabilization Yes - 1% TRITON-X-100
Fixative Paraformaldehyde
Username

Abcam user community

Verified customer

提交于 Mar 18 2015

Application Western blot
Loading amount 20 µg
Gel Running Conditions Reduced Denaturing
Sample Mouse Cell lysate - whole cell (HEPATOCYTES)
Specification HEPATOCYTES
Treatment 20 ug/ml poly(I:C)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Username

Abcam user community

Verified customer

提交于 Jul 09 2014

Application Western blot
Loading amount 100000 cells
Gel Running Conditions Reduced Denaturing (10%)
Sample Human Cell lysate - whole cell (Jurkats)
Specification Jurkats
Treatment TCR activation with OKT3 and anti CD28
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
Username

Dr. Tejpal Gill

Verified customer

提交于 Jan 13 2014

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Loading amount 50 µg
Gel Running Conditions Reduced Denaturing (8-12)
Sample Mouse Tissue lysate - whole (liver)
Specification liver
Blocking step No blocking step used for 30 minute(s) · Temperature: 30°C
Username

Dr. Gillian Hunter

Verified customer

提交于 Aug 06 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Loading amount 10 µg
Gel Running Conditions Reduced Denaturing
Sample Fruit fly (Drosophila melanogaster) Tissue lysate - whole (fly heart tube)
Specification fly heart tube
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: rt°C
Username

Abcam user community

Verified customer

提交于 Jul 02 2013

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