概述

  • 产品名称Anti-COUP TF1抗体[H8132]
    参阅全部 COUP TF1 一抗
  • 描述
    小鼠单克隆抗体[H8132] to COUP TF1
  • 特异性This antibody specifically recognizes human COUP TFI and cross reacts with mouse and rat COUP TF I. This antibody does not recognize human COUP TFII and EAR2.
  • 经测试应用适用于: Flow Cyt, WB, ELISA, IHC-Pmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    Immunized with Baculovirus-expressed Recombinant fragment: SSWRDPQDDV AGGNPGGPNP AAQAARGGGG GAGEQQQQAG SGAPHTPQTP GQPGAQ, corresponding to amino acids 6-81 of Human COUP TF1.

  • 阳性对照
    • Human Prostate gland, Rat Cerebrum.

性能

应用

Our Abpromise guarantee covers the use of ab41858 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Flow Cyt Use 1µg for 106 cells. ab170191-Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 48 kDa (predicted molecular weight: 46 kDa).
ELISA Use a concentration of 0.1 µg/ml.
IHC-P Use at an assay dependent concentration.

靶标

  • 功能Coup (chicken ovalbumin upstream promoter) transcription factor binds to the ovalbumin promoter and, in conjunction with another protein (S300-II) stimulates initiation of transcription. Binds to both direct repeats and palindromes of the 5'-AGGTCA-3' motif. Represses transcriptional activity of LHCG.
  • 序列相似性Belongs to the nuclear hormone receptor family. NR2 subfamily.
    Contains 1 nuclear receptor DNA-binding domain.
  • 细胞定位Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Chicken ovalbumin upstream promoter 1 antibody
    • COT1_HUMAN antibody
    • COUP transcription factor 1 antibody
    • COUP transcription factor I antibody
    • COUP-TF I antibody
    • COUP-TF1 antibody
    • EAR-3 antibody
    • EAR3 antibody
    • ERBAL3 antibody
    • NR2F1 antibody
    • NR2F2 antibody
    • Nuclear receptor subfamily 2 group F member 1 antibody
    • SVP44 antibody
    • TCFCOUP1 antibody
    • TFCOUP1 antibody
    • Transcription factor COUP 1 antibody
    • V ERBA related protein EAR 3 antibody
    • V-erbA-related protein 3 antibody
    see all

Anti-COUP TF1 antibody [H8132] 图像

  • ab41858 staining COUP TF1 in human prostate gland (10-50 ug/mL) by Immunohistochemistry, formalin-fixed paraffin embedded sections.
  • ab41858 staining COUP TF1 in rat cerebrum (10-50 ug/mL) by Immunohistochemistry, formalin-fixed paraffin embedded sections.
  • Anti-COUP TF1 antibody [H8132] (ab41858) at 1 µg/ml + Human brain tissue lysate - total protein (ab29466) at 10 µg

    Secondary
    Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 46 kDa
    Observed band size : 48 kDa (why is the actual band size different from the predicted?)


    Exposure time : 150 seconds
  • Overlay histogram showing SH-SY5Y cells stained with ab41858 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab41858, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

Anti-COUP TF1 antibody [H8132] (ab41858)参考文献

This product has been referenced in:
  • Alfano C  et al. COUP-TFI promotes radial migration and proper morphology of callosal projection neurons by repressing Rnd2 expression. Development 138:4685-97 (2011). Read more (PubMed: 21965613) »

See 1 Publication for this product

Product Wall

There are currently no Abreviews or Questions for ab41858.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"