Anti-COUP TF1抗体(ab96846)
Key features and details
- Rabbit polyclonal to COUP TF1
- Suitable for: IHC-P, WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术,可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-COUP TF1抗体
参阅全部 COUP TF1 一抗 -
描述
兔多克隆抗体to COUP TF1 -
宿主
Rabbit -
经测试应用
适用于: IHC-P, WB, ICC/IFmore details -
种属反应性
与反应: Human
预测可用于: Mouse, Rat, Cow -
免疫原
Synthetic peptide, corresponding to a sequence within amino acids 1-14 of Human COUP TF1 (NP_005645).
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阳性对照
- COUP TF1 transfected 293T lysate, HeLa cells and CA922 tissue.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 89.99% PBS, 10% Glycerol (glycerin, glycerine) -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab96846于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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IHC-P |
1/100 - 1/1000.
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WB |
1/500 - 1/3000. Predicted molecular weight: 46 kDa.
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ICC/IF |
1/100 - 1/200.
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说明 |
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IHC-P
1/100 - 1/1000. |
WB
1/500 - 1/3000. Predicted molecular weight: 46 kDa. |
ICC/IF
1/100 - 1/200. |
靶标
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功能
Coup (chicken ovalbumin upstream promoter) transcription factor binds to the ovalbumin promoter and, in conjunction with another protein (S300-II) stimulates initiation of transcription. Binds to both direct repeats and palindromes of the 5'-AGGTCA-3' motif. Represses transcriptional activity of LHCG. -
序列相似性
Belongs to the nuclear hormone receptor family. NR2 subfamily.
Contains 1 nuclear receptor DNA-binding domain. -
细胞定位
Nucleus. - Information by UniProt
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数据库链接
- Entrez Gene: 327684 Cow
- Entrez Gene: 7025 Human
- Entrez Gene: 13865 Mouse
- Entrez Gene: 81808 Rat
- Omim: 132890 Human
- SwissProt: Q9TTR8 Cow
- SwissProt: P10589 Human
- SwissProt: Q60632 Mouse
see all -
别名
- Chicken ovalbumin upstream promoter 1 antibody
- COT1_HUMAN antibody
- COUP transcription factor 1 antibody
see all
图片
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ab96846 at 1/100 dilution staining COUP TF1 in CA922 by Immunohistochemistry, Paraffin-embedded tissue.
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All lanes : Anti-COUP TF1 antibody (ab96846) at 1/500 dilution
Lane 1 : Non-transfected 293T lysate
Lane 2 : COUP TF1 transfected 293T lysate
Predicted band size: 46 kDa
12% SDS-PAGE. -
ab96846 at 1/200 dilution staining COUP TF in HeLa cells by Immunofluorescence, Paraformaldehyde fixed. Lower image is merged with DNA probe.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (2)
ab96846 被引用在 2 文献中.
- López-González L et al. Populational heterogeneity and partial migratory origin of the ventromedial hypothalamic nucleus: genoarchitectonic analysis in the mouse. Brain Struct Funct 228:537-576 (2023). PubMed: 36598560
- Fowler PA et al. In utero exposure to cigarette smoke dysregulates human fetal ovarian developmental signalling. Hum Reprod N/A:N/A (2014). IHC-P ; Human . PubMed: 24847019