Anti-COUP TF1抗体(ab80852)

概述

  • 产品名称Anti-COUP TF1抗体
    参阅全部 COUP TF1 一抗
  • 描述
    兔多克隆抗体to COUP TF1
  • 经测试应用适用于: WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Sheep, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Pig, Drosophila melanogaster, Zebrafish
  • 免疫原

    Synthetic peptide within residues AIVLFTSDAC GLSDAAHIES LQEKSQCALE EYVRSQYPNQ PSRFGKLLLR corresponding to C terminal amino acids 323-372 of human COUP TF1 (NP_005645).

  • 阳性对照
    • Transfected 293T cell lysate.

性能

应用

Our Abpromise guarantee covers the use of ab80852 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Detects a band of approximately 46 kDa (predicted molecular weight: 46 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

靶标

  • 功能Coup (chicken ovalbumin upstream promoter) transcription factor binds to the ovalbumin promoter and, in conjunction with another protein (S300-II) stimulates initiation of transcription. Binds to both direct repeats and palindromes of the 5'-AGGTCA-3' motif. Represses transcriptional activity of LHCG.
  • 序列相似性Belongs to the nuclear hormone receptor family. NR2 subfamily.
    Contains 1 nuclear receptor DNA-binding domain.
  • 细胞定位Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Chicken ovalbumin upstream promoter 1 antibody
    • COT1_HUMAN antibody
    • COUP transcription factor 1 antibody
    • COUP transcription factor I antibody
    • COUP-TF I antibody
    • COUP-TF1 antibody
    • EAR-3 antibody
    • EAR3 antibody
    • ERBAL3 antibody
    • NR2F1 antibody
    • NR2F2 antibody
    • Nuclear receptor subfamily 2 group F member 1 antibody
    • SVP44 antibody
    • TCFCOUP1 antibody
    • TFCOUP1 antibody
    • Transcription factor COUP 1 antibody
    • V ERBA related protein EAR 3 antibody
    • V-erbA-related protein 3 antibody
    see all

Anti-COUP TF1 antibody 图像

  • Anti-COUP TF1 antibody (ab80852) at 1 µg/ml (in 5% skim milk / PBS buffer) + Transfected 293T cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 46 kDa
    Observed band size : 46 kDa
    Additional bands at : 28 kDa. We are unsure as to the identity of these extra bands.

Anti-COUP TF1 antibody (ab80852)参考文献

ab80852 has not yet been referenced specifically in any publications.

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Zebrafish Cell (Whole mount)
Specification Whole mount
Fixative Paraformaldehyde
Permeabilization Yes - Acetone
Blocking step Serum as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 4°C
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提交于 Nov 11 2010

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