概述

  • 产品名称Anti-COUP TF1抗体
    参阅全部 COUP TF1 一抗
  • 描述
    兔多克隆抗体to COUP TF1
  • 经测试应用适用于: WB, ELISAmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Chicken, Cow, Pig, Zebrafish
  • 免疫原

    Synthetic peptide: GAGEQQQQAG SGAPHTPQTP GQPGAPATPG TAGDKGQGPP GSGQSQQHIE , corresponding to N terminal amino acids 36-85 of Human COUP TF1

  • 阳性对照
    • Transfected 293T cell lysate

性能

应用

Our Abpromise guarantee covers the use of ab80698 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Detects a band of approximately 50 kDa (predicted molecular weight: 46 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

ELISA titre using peptide based assay 1/312500.

靶标

  • 功能Coup (chicken ovalbumin upstream promoter) transcription factor binds to the ovalbumin promoter and, in conjunction with another protein (S300-II) stimulates initiation of transcription. Binds to both direct repeats and palindromes of the 5'-AGGTCA-3' motif. Represses transcriptional activity of LHCG.
  • 序列相似性Belongs to the nuclear hormone receptor family. NR2 subfamily.
    Contains 1 nuclear receptor DNA-binding domain.
  • 细胞定位Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Chicken ovalbumin upstream promoter 1 antibody
    • COT1_HUMAN antibody
    • COUP transcription factor 1 antibody
    • COUP transcription factor I antibody
    • COUP-TF I antibody
    • COUP-TF1 antibody
    • EAR-3 antibody
    • EAR3 antibody
    • ERBAL3 antibody
    • NR2F1 antibody
    • NR2F2 antibody
    • Nuclear receptor subfamily 2 group F member 1 antibody
    • SVP44 antibody
    • TCFCOUP1 antibody
    • TFCOUP1 antibody
    • Transcription factor COUP 1 antibody
    • V ERBA related protein EAR 3 antibody
    • V-erbA-related protein 3 antibody
    see all

Anti-COUP TF1 antibody 图像



  • Predicted band size : 46 kDa
    Western blot analysis of Human fetal lung tissue lysate labeling COUP TF1 with ab80698 at 1.0µg/ml.


  • Predicted band size : 46 kDa
    Western blot analysis of Human fetal brain tissue lysate labeling COUP TF1 with ab80698 at 1.0µg/ml.


  • Predicted band size : 46 kDa
    Western blot analysis of Human adult placenta tissue lysate labeling COUP TF1 with ab80698 at 1.0µg/ml.
  • Anti-COUP TF1 antibody (ab80698) at 1 µg/ml + transfected 293T cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 46 kDa
    Observed band size : 46 kDa
    Gel concentration 12%

Anti-COUP TF1 antibody (ab80698)参考文献

ab80698 has not yet been referenced specifically in any publications.

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