The COS7 nuclear extracts have been collected in Lysis Buffer after a 16 hour incubation with or without CoCl2 (0.15 mM).
The protein content has been determined by a Bradford based assay (2.50 mg/ml).
We recommend aliquoting the extracts into single use fractions and then storing.
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
These fibroblast-like cells grow in monolayers and are possible hosts for the propagation of pure populations of recombinant SV40 virus and contain T antigen of SV 40 virus. They are useful in virus replication studies and transfection studies.
has not yet been referenced specifically in any publications.