Cortisol ELISA试剂盒(ab108674)

概述

  • 产品名称Cortisol ELISA试剂盒
    参阅全部 Cortisol 试剂盒
  • 检测方法Colorimetric
  • 精确度
    批次内
    样品 n Mean SD CV%
    Urine 60 <=6.5%
    批次间
    样品 n Mean SD CV%
    Urine 30 <=7.2%
  • 测试
    1 x 96 test
  • 样品类型
    Urine
  • 检测类型Competitive
  • 灵敏度
    2.95 ng/ml
  • 范围
    10 ng/ml - 500 ng/ml
  • 复检

    107.48 %

  • 实验步骤Multiple steps standard assay
  • 种属反应性
    与反应: Human
  • 产品概述

    Abcam’s Cortisol in vitro competitive ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of Cortisol in urine.


    A 96-well plate has been precoated with anti-Cortisol. Samples and the Cortisol-HRP conjugate are added to the wells, where Cortisol in the sample competes with the added Cortisol-HRP for antibody binding. After incubation, the wells are washed to remove unbound material and TMB substrate is then added which is catalyzed by HRP to produce blue coloration. The reaction is terminated by addition of Stop Solution which stops the color development and produces a color change from blue to yellow. The intensity of signal is inversely proportional to the amount of Cortisol in the sample and the intensity is measured at 450 nm.

  • 经测试应用适用于: Competitive ELISAmore details
  • 平台Microplate

性能

  • 存放说明Store at +4°C. Please refer to protocols.
  • 组件 1 x 96 tests
    10X Wash Solution 1 x 50ml
    Anti-Cortisol IgG Coated Microplate (12 x 8 wells) 1 unit
    Cortisol Standard 0 - 0 ng/mL 1 x 1ml
    Cortisol Standard 1 - 10 ng/mL 1 x 1ml
    Cortisol Standard 2 - 50 ng/mL 1 x 1ml
    Cortisol Standard 3 - 150 ng/mL 1 x 1ml
    Cortisol Standard 4 – 500 ng/mL 1 x 1ml
    Cortisol-HRP Conjugate 1 x 33ml
    Cover foils 1 unit
    High control 1 x 1ml
    Low control 1 x 1ml
    Stop Solution 1 x 15ml
    Strip holder 1 unit
    TMB Substrate Solution 1 x 15ml
  • 研究领域

    应用

    Our Abpromise guarantee covers the use of ab108674 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    应用 Ab评论 说明
    Competitive ELISA Use at an assay dependent concentration.

    Cortisol ELISA Kit 图像

    • Representative Standard Curve using ab108674

    实验方案

    Cortisol ELISA Kit (ab108674)参考文献

    ab108674 has not yet been referenced specifically in any publications.

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    This product comes with full money back guarantee if used with human urine samples. Please reassure them if the product does not work they will get refund or replacements.

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