Anti-Cortisol Binding Globulin抗体(ab96270)


  • 产品名称Anti-Cortisol Binding Globulin抗体
    参阅全部 Cortisol Binding Globulin 一抗
  • 描述
    兔多克隆抗体to Cortisol Binding Globulin
  • 经测试应用适用于: WB, ICC/IF, IHC-Pmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Recombinant protein fragment corresponding to a region within amino acids 105 and 353 of Human Cortisol Binding Globulin (NP_001747)

  • 阳性对照
    • 293T, H1299, HeLa, HepG2, Molt-4,A549 and Raji cells. A431 whole cell lysate. IHC-P: Human liver FFPE tissue sections.


  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 10% Glycerol, 0.1M Tris, 0.1M Glycine, pH 7.0
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 克隆多克隆
  • 同种型IgG
  • 研究领域



Our Abpromise guarantee covers the use of ab96270 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500 - 1/3000. Predicted molecular weight: 45 kDa.
ICC/IF 1/100 - 1/200.
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.


  • 功能Major transport protein for glucocorticoids and progestins in the blood of almost all vertebrate species.
  • 组织特异性Plasma; synthesized in liver. Has also been identified in a number of glycocorticoid responsive cells.
  • 疾病相关Defects in SERPINA6 are a cause of corticosteroid-binding globulin deficiency (CBG deficiency) [MIM:611489]. CBG deficiency is an extremely rare hereditary disorder characterized by reduced corticosteroid-binding capacity with normal or low plasma corticosteroid-binding globulin concentration, and normal or low basal cortisol levels associated with hypo/hypertension and muscle fatigue.
  • 序列相似性Belongs to the serpin family.
  • 翻译后修饰N-glycosylated; binds 5 oligosaccharide chains.
    Glycosylation in position Asn-260 is needed for steroid binding.
  • 细胞定位Secreted.
  • Information by UniProt
  • 数据库链接
  • 别名
    • CBG antibody
    • CBG_HUMAN antibody
    • corticosteroid binding globulin antibody
    • Corticosteroid-binding globulin antibody
    • serine (or cysteine) proteinase inhibitor clade A (alpha 1 antiproteinase antitrypsin) member 6 antibody
    • Serpin A6 antibody
    • serpin peptidase inhibitor clade A (alpha 1 antiproteinase antitrypsin) member 6 antibody
    • Serpina6 antibody
    • Transcortin antibody
    see all

Anti-Cortisol Binding Globulin antibody 图像

  • Anti-Cortisol Binding Globulin antibody (ab96270) at 1/1000 dilution + A431 whole cell lysate at 30 µg

    Predicted band size : 45 kDa
  • Immunofluorescence analysis of paraformaldehyde-fixed A549 cells, using ab96270 at 1/200 dilution. Lower image merged with DNA probe.
  • IHC image of Cortisol Binding Globulin staining in human liver formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab96270, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Anti-Cortisol Binding Globulin antibody (ab96270)参考文献

ab96270 has not yet been referenced specifically in any publications.

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Abcam has not validated the combination of species/application used in this Abreview.
Application Immunoprecipitation
Sample Human Cell lysate - whole cell (A431 cell lysate)
Total protein in input 50 µg
Specification A431 cell lysate
Treatment Lysates pre-cleared with protein A sepharose
Immuno-precipitation step Protein A

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提交于 Jun 21 2012

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