Anti-Corticotropin Releasing Factor Receptor 2抗体(ab104368)

概述

  • 产品名称
    Anti-Corticotropin Releasing Factor Receptor 2抗体
    参阅全部 Corticotropin Releasing Factor Receptor 2 一抗
  • 描述
    兔多克隆抗体to Corticotropin Releasing Factor Receptor 2
  • 宿主
    Rabbit
  • 经测试应用
    适用于: WBmore details
  • 种属反应性
    与反应: Mouse, Rat
    预测可用于: Horse, Hamster, Cow, Dog, Human
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 50 - 150 of Rat Corticotropin Releasing Factor Receptor 2.

  • 阳性对照
    • This antibody gave a positive signal in the following tissue lysates: Rat Brain; Rat Forebrain; Rat Dorsal Root Ganglion; Mouse Dopaminergic Nucleus.

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液
    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituents: PBS, 1% BSA
    Note: Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
  • Concentration information loading...
  • 纯度
    Immunogen affinity purified
  • 克隆
    多克隆
  • 同种型
    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab104368 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Detects a band of approximately 51,54 kDa (predicted molecular weight: 48 kDa).

靶标

图片

  • All lanes : Anti-Corticotropin Releasing Factor Receptor 2 antibody (ab104368) at 1 µg/ml

    Lane 1 : Brain (Rat) Tissue Lysate
    Lane 2 : Rat Forebrain
    Lane 3 : Dorsal Root Ganglia (Rat) Tissue Lysate
    Lane 4 : Dopaminergic nucleus Mouse Tissue Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 48 kDa
    Observed band size: 51 + 54 kDa (why is the actual band size different from the predicted?)


    Exposure time: 30 seconds


    Corticotropin Releasing Factor Receptor 2 contains a number of potential glycosylation sites (SwissProt) which may explain its migration at a higher molecular weight than predicted. This antibody was raised against an immunogen that is predicted to recognize both alpha and beta isoforms of Corticotropin Releasing Factor Receptor 2. The predicted molecular weights of the alpha and beta isoforms are 48 kDa and 50 kDa respectively.

实验方案

文献

ab104368 has not yet been referenced specifically in any publications.

客户评价及客户问答

Application
Western blot
Sample
Human Tissue lysate - whole (intestinal tissue)
Gel Running Conditions
Reduced Denaturing (4-12% BIS TRIS)
Loading amount
28 µg
Specification
intestinal tissue
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Mr. Julien Chevalier

Verified customer

提交于 May 20 2015

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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