Anti-Corticotropin Releasing Factor Receptor 2抗体(ab104368)

概述

  • 产品名称Anti-Corticotropin Releasing Factor Receptor 2抗体
    参阅全部 Corticotropin Releasing Factor Receptor 2 一抗
  • 描述
    兔多克隆抗体to Corticotropin Releasing Factor Receptor 2
  • 经测试应用适用于: WBmore details
  • 种属反应性
    与反应: Mouse, Rat
    预测可用于: Horse, Hamster, Cow, Dog, Human
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 50 - 150 of Rat Corticotropin Releasing Factor Receptor 2.

  • 阳性对照
    • This antibody gave a positive signal in the following tissue lysates: Rat Brain; Rat Forebrain; Rat Dorsal Root Ganglion; Mouse Dopaminergic Nucleus.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituents: PBS, 1% BSA
    Note: Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab104368 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Detects a band of approximately 51,54 kDa (predicted molecular weight: 48 kDa).

靶标

Anti-Corticotropin Releasing Factor Receptor 2 antibody 图像

  • All lanes : Anti-Corticotropin Releasing Factor Receptor 2 antibody (ab104368) at 1 µg/ml

    Lane 1 : Brain (Rat) Tissue Lysate
    Lane 2 : Rat Forebrain
    Lane 3 : Dorsal Root Ganglia (Rat) Tissue Lysate
    Lane 4 : Dopaminergic nucleus Mouse Tissue Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 48 kDa
    Observed band size : 51 + 54 kDa (why is the actual band size different from the predicted?)


    Exposure time : 30 seconds

Anti-Corticotropin Releasing Factor Receptor 2 antibody (ab104368)参考文献

ab104368 has not yet been referenced specifically in any publications.

Product Wall

Application Western blot
Sample Human Tissue lysate - whole (intestinal tissue)
Gel Running Conditions Reduced Denaturing (4-12% BIS TRIS)
Loading amount 28 µg
Specification intestinal tissue
Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Mr. Julien Chevalier

Verified customer

提交于 May 20 2015

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"