Anti-CRHR2抗体(ab104368)
Key features and details
- Rabbit polyclonal to CRHR2
- Suitable for: WB
- Reacts with: Mouse, Rat
- Isotype: IgG
概述
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产品名称
Anti-CRHR2抗体
参阅全部 CRHR2 一抗 -
描述
兔多克隆抗体to CRHR2 -
宿主
Rabbit -
经测试应用
适用于: WBmore details -
种属反应性
与反应: Mouse, Rat
预测可用于: Horse, Hamster, Cow, Dog, Human -
免疫原
Synthetic peptide corresponding to Rat CRHR2 aa 50-150 conjugated to keyhole limpet haemocyanin.
(Peptide available asab125890) -
阳性对照
- This antibody gave a positive signal in the following tissue lysates: Rat Brain; Rat Forebrain; Rat Dorsal Root Ganglion; Mouse Dopaminergic Nucleus.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 1% BSA, PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab104368于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB | (1) |
Use a concentration of 1 µg/ml. Detects a band of approximately 51,54 kDa (predicted molecular weight: 48 kDa).
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说明 |
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WB
Use a concentration of 1 µg/ml. Detects a band of approximately 51,54 kDa (predicted molecular weight: 48 kDa). |
靶标
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功能
This is a receptor for corticotropin releasing factor. Shows high-affinity CRF binding. Also binds to urocortin I, II and III. The activity of this receptor is mediated by G proteins which activate adenylyl cyclase. -
序列相似性
Belongs to the G-protein coupled receptor 2 family. -
细胞定位
Cell membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 521831 Cow
- Entrez Gene: 482384 Dog
- Entrez Gene: 100055029 Horse
- Entrez Gene: 1395 Human
- Entrez Gene: 12922 Mouse
- Entrez Gene: 64680 Rat
- Omim: 602034 Human
- SwissProt: Q13324 Human
see all -
别名
- corticotropin releasing factor receptor antibody
- Corticotropin releasing hormone receptor 2 antibody
- Corticotropin-releasing factor receptor 2 antibody
see all
图片
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All lanes : Anti-CRHR2 antibody (ab104368) at 1 µg/ml
Lane 1 : Brain (Rat) Tissue Lysate
Lane 2 : Rat Forebrain
Lane 3 : Dorsal Root Ganglia (Rat) Tissue Lysate
Lane 4 : Dopaminergic nucleus Mouse Tissue Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 48 kDa
Observed band size: 51,54 kDa why is the actual band size different from the predicted?
Exposure time: 30 secondsCRHR2 contains a number of potential glycosylation sites (SwissProt) which may explain its migration at a higher molecular weight than predicted. This antibody was raised against an immunogen that is predicted to recognize both alpha and beta isoforms of Corticotropin Releasing Factor Receptor 2. The predicted molecular weights of the alpha and beta isoforms are 48 kDa and 50 kDa respectively.
数据表及文件
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SDS download
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Datasheet download
文献 (4)
ab104368 被引用在 4 文献中.
- Lovell AJ et al. The effects of diet and chronic exercise on skeletal muscle ghrelin response. Metabol Open 14:100182 (2022). PubMed: 35340718
- Hucik B et al. Regulation of adipose tissue lipolysis by ghrelin is impaired with high-fat diet feeding and is not restored with exercise. Adipocyte 10:338-349 (2021). PubMed: 34224298
- Cervone DT et al. Unacylated ghrelin stimulates fatty acid oxidation to protect skeletal muscle against palmitate-induced impairment of insulin action in lean but not high-fat fed rats. Metabol Open 5:100026 (2020). PubMed: 32812929
- Castillero E et al. Structural and functional cardiac profile after prolonged duration of mechanical unloading: potential implications for myocardial recovery. Am J Physiol Heart Circ Physiol 315:H1463-H1476 (2018). PubMed: 30141986