Anti-Corticotropin Releasing Factor抗体(ab8901)

概述

  • 产品名称Anti-Corticotropin Releasing Factor抗体
    参阅全部 Corticotropin Releasing Factor 一抗
  • 描述
    兔多克隆抗体to Corticotropin Releasing Factor
  • 经测试应用适用于: ICC, RIA, WB, IHC-Pmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide, corresponding to amino acids 1-41 of Rat Corticotropin Releasing Factor.

性能

应用

Our Abpromise guarantee covers the use of ab8901 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
AP Use at an assay dependent concentration.
ICC 1/1000.
RIA 1/600000.
WB 1/10000.
IHC-P 1/200 - 1/600.

靶标

  • 相关性CRF precursor is a 41-amino acid peptide derived from a 196-amino acid preprohormone. CRF is a neuroendocrine hormone secreted by the paraventricular nucleus (PVN) of the hypothalamus and is a key player in the hypothalamic pituitary adrenal axis. It is also synthesized in peripheral tissues, such as T lymphocytes and is highly expressed in the placenta. Neuronal CRF is important in a variety of diverse roles including, memory, depression, stress, kidney function, parturition, metabolism and feeding (to mention a few).
  • 细胞定位Cytoplasmic and Secreted
  • 数据库链接
  • 别名
    • Corticoliberin antibody
    • Corticotropin releasing factor antibody
    • Corticotropin releasing hormone antibody
    • CRF antibody
    • Crh antibody
    • CRH1 antibody
    see all

Anti-Corticotropin Releasing Factor antibody 图像

  • Anti-Corticotropin Releasing Factor antibody (ab8901) at 1/2500 dilution + Mouse hypothalamus whole tissue lysate at 50 µg

    Secondary
    HRP-conjugated Donkey anti-rabbit IgG polyclonal - undiluted
    Developed using the ECL technique

    Performed under non-reducing conditions.

    Observed band size : 25 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 17 kDa (possible non-specific binding),23 kDa (possible non-specific binding),35 kDa (possible non-specific binding).

    Exposure time : 1 minute

    This image is courtesy of an Abreview submitted by Jean-Christophe Helbling

    See Abreview

  • Radioimmunoassay using ab8901
    - Day 1: Add Recombinant Protein A/G (10ug/ml) to a 96 wells plate; parafilm wrap; incubate overnight at 4°C.
    - Day 2: Aspirate and wash the plate to remove loosely bound Protein A/G; add antibody solution (dilution 1:50000); parafilm wrap; incubate 24h at 4°C.
    - Day 3: Aspirate and wash the plate to remove loosely bound antibody; add CRF standard (0, 0.03, 0.1, 0.3, 1, 3, 10, 30 ng/ml); parafilm wrap; incubate 24h at 4°C.
    - Day 4: DO NOT ASPIRATE; add 125I-CRF; incubate 24h at 4°C.
    - Day 5: Aspirate and wash the plate; break the plate and collect the wells in scintillation tubes; add 2ml of scintillation liquid; count in beta counter (open the 3H window to at least 30-40 keV), at least 2min/tube.
  • Immunohistochemical analysis of rat brain tissue, staining Corticotropin Releasing Factor with ab8901.

    Tissue was fixed with paraformaldehyde, permeabilized with 0.01% Triton X-100 and blocked with 10% serum for 1 hour at 25°C; antigen retrieval was by heat mediation in a citrate buffer (pH 6). Samples were incubated with primary antibody (1/100 in diluent) for 24 hours at 4°C. An AlexaFluor®594-conjugated goat anti-rabbit polyclonal IgG (1/500) was used as the secondary antibody. The figure on the left is a secondary only control.

    See Abreview

Anti-Corticotropin Releasing Factor antibody (ab8901)参考文献

This product has been referenced in:
  • Feetham CH  et al. TRPV4 and K(Ca) ion channels functionally couple as osmosensors in the paraventricular nucleus. Br J Pharmacol 172:1753-68 (2015). Read more (PubMed: 25421636) »
  • Cuevas Guaman M  et al. Administration of antenatal glucocorticoids and postnatal surfactant ameliorates respiratory distress syndrome-associated neonatal lethality in Erk3(-/-) mouse pups. Pediatr Res 76:24-32 (2014). IHC ; Mouse . Read more (PubMed: 24732107) »

See all 7 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Loading amount 50 µg
Gel Running Conditions Non-reduced Denaturing
Sample Mouse Tissue lysate - whole (Hypothalamus)
Specification Hypothalamus
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Mr. Jean-christophe Helbling

Verified customer

提交于 Sep 25 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Rat Tissue sections (Brain)
Specification Brain
Fixative Paraformaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citrate
Permeabilization Yes - 0.01% Triton-X
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 Jan 04 2013

Thank you for sending us your customer's questionnaire and for contacting us. I am sorry to hear this antibody is not providing satisfactory results.

The details provided will enable us to investigate this case and will provide us with vital...

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Thank you for contacting us.


The link to our conjugation kits and purifications kits is either http://www.abcam.com/EasyLink or
http://www.abcam.com/index.html?pageconfig=resource&rid=13148&source=pagetrap&viapagetrap=e...

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Thank you for your reply. I have published your Abreview on our online datasheet and awarded Abpoints to your user account. You can view and redeem your Abpoints by logging in to your account on our website.

Additionally, since you have submi...

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Thank you for contacting Abcam regarding ab8901.


The predicted molecular weight of this protein is 21kDa. In WB you should detect a band at approximately this size.


I hope this information is helpful. Please do not hesit...

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I am very pleased to hear you would like to use ab8901 in RIA and send us your data and an image of your results.

This code will give you 1 freeprimary antibody before the expiration date. To redeem this offer, please submit an Abreview with...

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Thank you for your reply.

I do apologize as I have been unable to obtain any more specific information regarding the dilution of this antibody to be used in RIA.

Due to this lack of specific details, I would like to offer you a te...

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Thank you for contacting Abcam regarding ab8901.


I have confirmed with the laboratory that RIA was not tested in house, but based on the following references:

1. Widmaier EP, Lim AT, Vale W. (1989) Secretion of corticotropin-...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Rat Tissue sections (Brain)
Specification Brain
Fixative Paraformaldehyde
Antigen retrieval step None
Permeabilization Yes - 0.1% triton X-100
Blocking step Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Username

Abcam user community

Verified customer

提交于 Feb 17 2009

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"