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Our Abpromise guarantee covers the use of ab8901 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|AP||Use at an assay dependent concentration.|
|IHC-P||1/200 - 1/600.|
This image is courtesy of an Abreview submitted by Jean-Christophe Helbling
Ab8901 staining Corticotropin releasing factor in Human placenta tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 2% BSA for 10 minutes at 21°C; antigen retrieval was by heat mediation in a citrate buffer. Samples were incubated with primary antibody (1/4000 in blocking buffer) for 16 hours at 21°C. A diluted Biotin-conjugated Goat anti-rabbit IgG polyclonal (1/300) was used as the secondary antibody.