使用敲除细胞株进行验证RabMAb

Anti-Cortactin抗体[EPR1923Y] (ab68438)

概述

  • 产品名称Anti-Cortactin抗体[EPR1923Y]
    参阅全部 Cortactin 一抗
  • 描述
    兔单克隆抗体[EPR1923Y] to Cortactin
  • 经测试应用适用于: WB, ICC, Flow Cytmore details
    不适用于: IHC-P or IP
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    A synthetic peptide corresponding to residues near the C-terminus of human Cortactin.

  • 阳性对照
    • C6, MCF-7, Hela and A431 cell lysate and permeabilized MCF-7 cells.
  • 常规说明

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

性能

相关产品

应用

Our Abpromise guarantee covers the use of ab68438 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB
ICC
Flow Cyt
  • 应用说明Flow Cyt: 1/30.
    ICC: 1/100 - 1/250.
    WB: 1/5000. Detects bands of approximately 75 and 80 kDa (predicted molecular weight: 80 kDa).

    Is unsuitable for IHC-P or IP.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 功能Contributes to the organization of the actin cytoskeleton and cell structure. Plays a role in the regulation of cell migration. Plays a role in the invasiveness of cancer cells, and the formation of metastases.
    • 序列相似性Contains 7 cortactin repeats.
      Contains 1 SH3 domain.
    • 结构域The SH3 motif may mediate binding to the cytoskeleton.
    • 翻译后修饰Tyrosine phosphorylation in transformed cells may contribute to cellular growth regulation and transformation.
    • 细胞定位Cytoplasm > cytoskeleton. Cell projection > lamellipodium. Cell projection > ruffle. Associated with membrane ruffles and lamellipodia.
    • Information by UniProt
    • 数据库链接
    • 别名
      • Amplaxin antibody
      • CTTN antibody
      • EMS 1 antibody
      • EMS1 antibody
      • FLJ34459 antibody
      • Mammary tumor and squamous cell carcinoma associated antibody
      • Oncogene EMS1 antibody
      • p80/85 src substrate antibody
      • Src substrate cortactin antibody
      • SRC8_HUMAN antibody
      see all

    Anti-Cortactin antibody [EPR1923Y] 图像



    • Predicted band size : 80 kDa

      Lane 1: Wild-type HAP1 cell lysate (40 µg)
      Lane 2: Cortactin knockout HAP1 cell lysate (40 µg)
      Lane 3: NIH3T3 cell lysate (20 µg)
      Lane 4: (20 µg)
      Lanes 1 - 4: Merged signal (red and green). Green - ab68438 observed at 70 kDa. Red - loading control, ab8245, observed at 37 kDa.

      ab68438 was shown to recognize Cortactin  when Cortactin  knockout samples were used, along with additional cross-reactive bands. Wild-type and Cortactin  knockout samples were subjected to SDS-PAGE. Ab68438 and ab8245 (loading control to GAPDH) were diluted at 1/1000 and 1:10,000 dilution respectively and incubated overnight at 4C. Blots were developed with IRDye® 800CW Goat anti-Rabbit IgG (H + L) and IRDye® 680 Goat anti-Mouse IgG (H + L) secondary antibodies at 1:10,000 dilution for 1 hour at room temperature before imaging.

    • All lanes : Anti-Cortactin antibody [EPR1923Y] (ab68438) at 1/5000 dilution

      Lane 1 : C6 cell lysate
      Lane 2 : MCF-7 cell lysate
      Lane 3 : Hela cell lysate
      Lane 4 : A431 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size : 80 kDa
      Observed band size : 80 kDa
      Additional bands at : 75 kDa. We are unsure as to the identity of these extra bands.
    • Flow cytometric analysis of permeabilized MCF-7 cells using ab68438 1:30 dilution (pink) or a rabbit IgG (negative) (green).

    Anti-Cortactin antibody [EPR1923Y] (ab68438)参考文献

    ab68438 has not yet been referenced specifically in any publications.

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