重组Anti-Cortactin抗体[EP1922Y] (ab81208)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP1922Y] to Cortactin
- Suitable for: Flow Cyt (Intra), WB, IHC-P, ICC/IF, IP
- Knockout validated
- Reacts with: Mouse, Human
Related conjugates and formulations
概述
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产品名称
Anti-Cortactin抗体[EP1922Y]
参阅全部 Cortactin 一抗 -
描述
兔单克隆抗体[EP1922Y] to Cortactin -
宿主
Rabbit -
经测试应用
适用于: Flow Cyt (Intra), WB, IHC-P, ICC/IF, IPmore details -
种属反应性
与反应: Mouse, Human
预测可用于: Rat -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- WB: HeLa and HEK-293T cell lysate. IHC-P: Human breast carcinoma. ICC/IF: MCF7 and wild-type HAP1 cells. IP: HeLa cell lysate
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EP1922Y -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
- Alexa Fluor® 488 Anti-Cortactin antibody [EP1922Y] (ab202648)
- Alexa Fluor® 647 Anti-Cortactin antibody [EP1922Y] (ab202650)
- PE Anti-Cortactin antibody [EP1922Y] (ab209758)
- Alexa Fluor® 555 Anti-Cortactin antibody [EP1922Y] (ab215298)
- Alexa Fluor® 594 Anti-Cortactin antibody [EP1922Y] (ab215300)
- Anti-Cortactin antibody [EP1922Y] - BSA and Azide free (ab223125)
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Compatible Secondaries
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Isotype control
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KO cell lines
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KO cell lysates
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab81208于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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Flow Cyt (Intra) |
1/10.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
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WB |
1/50000 - 1/100000. Predicted molecular weight: 62 kDa.
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IHC-P |
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Heat mediated antigen retrieval with citrate buffer, pH 6 or Tris-EDTA, pH 9 are both suitable methods IHC-P with this antibody. |
|
ICC/IF | (2) |
1/1000.
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IP |
1/50.
|
说明 |
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Flow Cyt (Intra)
1/10. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
WB
1/50000 - 1/100000. Predicted molecular weight: 62 kDa. |
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Heat mediated antigen retrieval with citrate buffer, pH 6 or Tris-EDTA, pH 9 are both suitable methods IHC-P with this antibody. |
ICC/IF
1/1000. |
IP
1/50. |
靶标
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功能
Contributes to the organization of the actin cytoskeleton and cell structure. Plays a role in the regulation of cell migration. Plays a role in the invasiveness of cancer cells, and the formation of metastases. -
序列相似性
Contains 7 cortactin repeats.
Contains 1 SH3 domain. -
结构域
The SH3 motif may mediate binding to the cytoskeleton. -
翻译后修饰
Tyrosine phosphorylation in transformed cells may contribute to cellular growth regulation and transformation. -
细胞定位
Cytoplasm > cytoskeleton. Cell projection > lamellipodium. Cell projection > ruffle. Associated with membrane ruffles and lamellipodia. - Information by UniProt
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数据库链接
- Entrez Gene: 2017 Human
- Entrez Gene: 13043 Mouse
- Entrez Gene: 60465 Rat
- GenBank: NM_138565 Human
- GenBank: NM_007803 Mouse
- Omim: 164765 Human
- SwissProt: Q14247 Human
- SwissProt: Q60598 Mouse
see all -
别名
- Amplaxin antibody
- CTTN antibody
- EMS 1 antibody
see all
图片
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All lanes : Anti-Cortactin antibody [EP1922Y] (ab81208) at 1/50000 dilution
Lane 1 : Wild-type HEK-293T cell lysate
Lane 2 : CTNN knockout HEK-293T cell lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 62 kDa
Observed band size: 70 kDa why is the actual band size different from the predicted?Lanes 1-2: Merged signal (red and green). Green - ab81208 observed at 70 kDa. Red - loading control ab8245 observed at 37 kDa.
ab81208 Anti-Cortactin antibody [EP1922Y] was shown to specifically react with Cortactin in wild-type HEK-293T cells. Loss of signal was observed when knockout cell line ab266819 (knockout cell lysate ab257147) was used. Wild-type and Cortactin knockout samples were subjected to SDS-PAGE. ab81208 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 50000 Dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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ab81208 staining Cortactin in wild-type HAP1 cells (top panel) and Cortactin knockout HAP1 cells (bottom panel). The cells were fixed with 4% formaldehyde (10min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated with ab81208 at 1/1000 dilution and ab195889 at 1/250 dilution (shown in pseudo colour red) overnight at +4°C, followed by a further incubation at room temperature for 1h with a goat secondary antibody to Rabbit IgG (Alexa Fluor® 488) (ab150081) at 2 μg/ml (shown in green). Nuclear DNA was labelled in blue with DAPI.
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
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All lanes : Anti-Cortactin antibody [EP1922Y] (ab81208) at 1/2000 dilution
Lane 1 : Wild-type HAP1 cell lysate
Lane 2 : Cortactin knockout HAP1 cell lysate
Lane 3 : NIH3T3 cell lysate
Lane 4 : A431 cell lysate
Lysates/proteins at 20 µg per lane.
Predicted band size: 62 kDaLanes 1 - 4: Merged signal (red and green). Green - ab81208 observed at 62 kDa. Red - loading control, ab8245, observed at 37 kDa.
ab81208 was shown to specifically react with Cortactin in wild-type HAP1 cells along with additional cross reactive bands. No band was observed when Cortactin knockout samples were examined. Wild-type and Cortactin knockout samples were subjected to SDS-PAGE. ab81208 and ab8245 (loading control to GAPDH) were both diluted 1/2000 and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10,000 dilution for 1 hour at room temperature before imaging.
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Purified ab81208 at 1/50 dilution (2µg) immunoprecipitating Cortactin in HeLa whole cell lysate.
Lane 1 (input): HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate 10µg
Lane 2 (+): ab81208 + HeLa whole cell lysate.
Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab81208 in HeLa whole cell lysate.
VeriBlot for IP Detection Reagent (HRP) (ab131366) (1/1000 dilution) was used for Western blotting.
Blocking Buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM/TBST.
Observed band size: 62 kDa -
Anti-Cortactin antibody [EP1922Y] (ab81208) at 1/100000 dilution + HeLa cell lysate at 10 µg
Secondary
HRP labelled Goat anti-Rabbit at 1/2000 dilution
Predicted band size: 62 kDa -
Immunohistochemical analysis of paraffin-embedded human breast carcinoma staining Cortacin with ab81208 at 1/100 dilution. Heat mediated antigen retrieval with Tris-EDTA (pH 9) was perfomed.
Heat mediated antigen retrieval was performed with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunofluorescent staining of MCF7 cells using 1/100 ab81208
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Overlay histogram showing HeLa cells stained with ab81208 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab81208, 1/100 dilution) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (32)
ab81208 被引用在 32 文献中.
- Asada Y et al. Comparison of the wound-healing efficacy of gelatin sponge dressings and that of artificial dermis using atelocollagen in a rat cranial periosteal defect model. Exp Anim 71:161-172 (2022). PubMed: 34789616
- Brandolini L et al. Paclitaxel binds and activates C5aR1: A new potential therapeutic target for the prevention of chemotherapy-induced peripheral neuropathy and hypersensitivity reactions. Cell Death Dis 13:500 (2022). PubMed: 35614037
- Iwamuro M et al. Laminin 511-E8 Fragment Offers Superior Adhesion Properties for Gastric Cancer Cells Compared with Full-Length Laminin 511. Curr Issues Mol Biol 44:1539-1551 (2022). PubMed: 35723363
- Iwahara N et al. Activation of SIRT1 promotes membrane resealing via cortactin. Sci Rep 12:15328 (2022). PubMed: 36097021
- Mondal C et al. A proliferative to invasive switch is mediated by srGAP1 downregulation through the activation of TGF-β2 signaling. Cell Rep 40:111358 (2022). PubMed: 36130489