概述

  • 产品名称Anti-Cortactin抗体
    参阅全部 Cortactin 一抗
  • 描述
    兔多克隆抗体to Cortactin
  • 经测试应用适用于: IHC-P, WB, IPmore details
  • 种属反应性
    与反应: Human
    预测可用于: Gorilla, Orangutan
  • 免疫原

    Synthetic peptide corresponding to a region within amino acids 450-500 of Human Cortactin (NP_005222.2).

  • 阳性对照
    • Whole cell lysate from HeLa cells and 293T cells

性能

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应用

Our Abpromise guarantee covers the use of ab93796 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P 1/200 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/2000 - 1/10000. Predicted molecular weight: 62 kDa.
IP Use at 2-5 µg/mg of lysate.

靶标

  • 功能Contributes to the organization of the actin cytoskeleton and cell structure. Plays a role in the regulation of cell migration. Plays a role in the invasiveness of cancer cells, and the formation of metastases.
  • 序列相似性Contains 7 cortactin repeats.
    Contains 1 SH3 domain.
  • 结构域The SH3 motif may mediate binding to the cytoskeleton.
  • 翻译后修饰Tyrosine phosphorylation in transformed cells may contribute to cellular growth regulation and transformation.
  • 细胞定位Cytoplasm > cytoskeleton. Cell projection > lamellipodium. Cell projection > ruffle. Associated with membrane ruffles and lamellipodia.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Amplaxin antibody
    • CTTN antibody
    • EMS 1 antibody
    • EMS1 antibody
    • FLJ34459 antibody
    • Mammary tumor and squamous cell carcinoma associated antibody
    • Oncogene EMS1 antibody
    • p80/85 src substrate antibody
    • Src substrate cortactin antibody
    • SRC8_HUMAN antibody
    see all

Anti-Cortactin antibody 图像

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human prostate carcinoma tissue labelling Cortactin with ab93796 at 1/1000 (0.2µg/ml). Detection: DAB.
  • All lanes : Anti-Cortactin antibody (ab93796) at 0.04 µg/ml

    Lane 1 : Whole cell lysate from HeLa cells at 50 µg
    Lane 2 : Whole cell lysate from HeLa cells at 15 µg
    Lane 3 : Whole cell lysate from HeLa cells at 5 µg
    Lane 4 : Whole cell lysate from 293T cells at 50 µg

    Developed using the ECL technique

    Predicted band size : 62 kDa


    Exposure time : 10 seconds
  • Detection of Cortactin in Immunoprecipitates of Whole cell lysate from HeLa cells (1 mg for IP, 20% of IP loaded) using ab93796 at 3 µg/mg lysate for IP (Lane 1) and at 0.4 µg/ml for subsequent Western blot detection. Lane 2 represents control IgG IP. Detection: Chemiluminescence with an exposure time of 1 seconds.

Anti-Cortactin antibody (ab93796)参考文献

ab93796 has not yet been referenced specifically in any publications.

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