概述

  • 产品名称Anti-Cortactin抗体
    参阅全部 Cortactin 一抗
  • 描述
    兔多克隆抗体to Cortactin
  • 特异性ab51101 detects endogenous levels of total Cortactin protein.
  • 经测试应用适用于: WB, ELISAmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat
  • 免疫原

    A synthetic non-phosphopeptide derived from human Cortactin around the phosphorylation site of tyrosine 466 (P-V-YP-E-T).

  • 阳性对照
    • Cos-7 cell extracts

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • 存储溶液Preservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 纯化说明ab51101 affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab51101 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500 - 1/1000. Detects a band of approximately 70 kDa (predicted molecular weight: 80 kDa).
ELISA 1/20000.

靶标

  • 功能Contributes to the organization of the actin cytoskeleton and cell structure. Plays a role in the regulation of cell migration. Plays a role in the invasiveness of cancer cells, and the formation of metastases.
  • 序列相似性Contains 7 cortactin repeats.
    Contains 1 SH3 domain.
  • 结构域The SH3 motif may mediate binding to the cytoskeleton.
  • 翻译后修饰Tyrosine phosphorylation in transformed cells may contribute to cellular growth regulation and transformation.
  • 细胞定位Cytoplasm > cytoskeleton. Cell projection > lamellipodium. Cell projection > ruffle. Associated with membrane ruffles and lamellipodia.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Amplaxin antibody
    • CTTN antibody
    • EMS 1 antibody
    • EMS1 antibody
    • FLJ34459 antibody
    • Mammary tumor and squamous cell carcinoma associated antibody
    • Oncogene EMS1 antibody
    • p80/85 src substrate antibody
    • Src substrate cortactin antibody
    • SRC8_HUMAN antibody
    see all

Anti-Cortactin antibody 图像

  • All lanes : Anti-Cortactin antibody (ab51101) at 1/500 dilution

    Lane 1 : Cos-7 cell extract
    Lane 2 : Cos-7 cell extract and immunizing peptide


    Predicted band size : 80 kDa
    Observed band size : 70 kDa (why is the actual band size different from the predicted?)

Anti-Cortactin antibody (ab51101)参考文献

ab51101 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab51101.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"